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Bottlebrush copolymers for gene delivery: influence of architecture, charge density, and backbone length on transfection efficiency.
Floyd, Thomas G; Song, Ji-Inn; Hapeshi, Alexia; Laroque, Sophie; Hartlieb, Matthias; Perrier, Sébastien.
Afiliación
  • Floyd TG; Department of Chemistry, University of Warwick, Gibbet Hill Road, Coventry, CV4 7AL, UK. s.perrier@warwick.ac.uk.
  • Song JI; Department of Chemistry, University of Warwick, Gibbet Hill Road, Coventry, CV4 7AL, UK. s.perrier@warwick.ac.uk.
  • Hapeshi A; Department of Chemistry, University of Warwick, Gibbet Hill Road, Coventry, CV4 7AL, UK. s.perrier@warwick.ac.uk.
  • Laroque S; Department of Chemistry, University of Warwick, Gibbet Hill Road, Coventry, CV4 7AL, UK. s.perrier@warwick.ac.uk.
  • Hartlieb M; Institute of Chemistry, University of Potsdam, Karl-Liebknecht-Straße 24-25, 14476, Potsdam, Germany. mhartlieb@uni-potsdam.de.
  • Perrier S; Fraunhofer Institute for Applied Polymer Research (IAP), Geiselbergstraße 69, Potsdam-Golm, Germany.
J Mater Chem B ; 10(19): 3696-3704, 2022 05 18.
Article en En | MEDLINE | ID: mdl-35441653
The influence of polymer architecture of polycations on their ability to transfect mammalian cells is probed. Polymer bottle brushes with grafts made from partially hydrolysed poly(2-ethyl-2-oxazoline) are used while varying the length of the polymer backbone as well as the degree of hydrolysis (cationic charge content). Polyplex formation is investigated via gel electrophoresis, dye-displacement and dynamic light scattering. Bottle brushes show a superior ability to complex pDNA when compared to linear copolymers. Also, nucleic acid release was found to be improved by a graft architecture. Polyplexes based on bottle brush copolymers showed an elongated shape in transmission electron microscopy images. The cytotoxicity against mammalian cells is drastically reduced when a graft architecture is used instead of linear copolymers. Moreover, the best-performing bottle brush copolymer showed a transfection ability comparable with that of linear poly(ethylenimine), the gold standard of polymeric transfection agents, which is used as positive control. In combination with their markedly lowered cytotoxicity, cationic bottle brush copolymers are therefore shown to be a highly promising class of gene delivery vectors.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polímeros / Técnicas de Transferencia de Gen Aspecto: Implementation_research Límite: Animals Idioma: En Revista: J Mater Chem B Año: 2022 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polímeros / Técnicas de Transferencia de Gen Aspecto: Implementation_research Límite: Animals Idioma: En Revista: J Mater Chem B Año: 2022 Tipo del documento: Article Pais de publicación: Reino Unido