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Immunomodulatory Effect and an Intervention of TNF Signalling Leading to Apoptotic and Cell Cycle Arrest on ORL-204 Oral Cancer Cells by Tiger Milk Mushroom, Lignosus rhinocerus.
Yap, Hui Yeng Yeannie; Kong, Boon Hong; Yap, Chee Sum Alvin; Ong, Kien Chai; Zain, Rosnah Binti; Tan, Soon Hao; Zaini, Zuraiza Mohamad; Ng, Szu Ting; Tan, Chon Seng; Fung, Shin Yee.
Afiliación
  • Yap HYY; Department of Oral Biology and Biomedical Sciences, Faculty of Dentistry, MAHSA University, Jalan SP 2, Bandar Saujana Putra, 42610 Jenjarom, Selangor, Malaysia.
  • Kong BH; Medicinal Mushroom Research Group (MMRG), Department of Molecular Medicine, Faculty of Medicine, Universiti Malaya, 50603 Kuala Lumpur, Malaysia.
  • Yap CSA; Medicinal Mushroom Research Group (MMRG), Department of Molecular Medicine, Faculty of Medicine, Universiti Malaya, 50603 Kuala Lumpur, Malaysia.
  • Ong KC; Department of Biomedical Sciences, Faculty of Medicine, Universiti Malaya, 50603 Kuala Lumpur, Malaysia.
  • Zain RB; Department of Oral Pathology and Oral Medicine, Faculty of Dentistry, MAHSA University, Jalan SP 2, Bandar Saujana Putra, 42610 Jenjarom, Selangor, Malaysia.
  • Tan SH; Oral Cancer Research and Coordinator (OCRCC), Faculty of Dentistry, Universiti Malaya, 50603 Kuala Lumpur, Malaysia.
  • Zaini ZM; Department of Oral and Maxillofacial Clinical Sciences, Faculty of Dentistry, Universiti Malaya, 50603 Kuala Lumpur, Malaysia.
  • Ng ST; Department of Biomedical Sciences, Faculty of Medicine, Universiti Malaya, 50603 Kuala Lumpur, Malaysia.
  • Tan CS; Oral Cancer Research and Coordinator (OCRCC), Faculty of Dentistry, Universiti Malaya, 50603 Kuala Lumpur, Malaysia.
  • Fung SY; Department of Oral and Maxillofacial Clinical Sciences, Faculty of Dentistry, Universiti Malaya, 50603 Kuala Lumpur, Malaysia.
Food Technol Biotechnol ; 60(1): 80-88, 2022 Mar.
Article en En | MEDLINE | ID: mdl-35440880
Research background: Tiger milk mushroom (Lignosus rhinocerus) is a medicinal mushroom that is geographically distributed in the region of South China, Thailand, Malaysia, Indonesia, Philippines and Papua New Guinea. Consumption of its sclerotium has been reported to treat various ailments. However, its anticancer potential towards oral cancer cell lines is yet to be determined considering the traditional method of its consumption by biting/chewing of the sclerotium. Experimental approach: Mushroom sclerotial powder of cultivar TM02® was extracted and fractionated in a chromatographic column prior to cytotoxicity testing against a panel of human oral cancer cell lines. The capability of the identified bioactive fraction in regulating several molecules associated with its tumour necrosis factor (TNF) pathway was investigated. Results and conclusions: 2,5-Diphenyl-2H-tetrazolium bromide (MTT) proliferation assay indicated that cell lines ORL-48 (derived from gingiva), ORL-188 (derived from the tongue) and ORL-204 (derived from buccal mucosa) were inhibited by cold water extract of L. rhinocerus sclerotia and its high-molecular-mass fraction (HMM) in varying degrees with ORL-204 being most affected. Hence, the treatment of ORL-204 with HMM mushroom extract was further investigated. HMM mushroom extract induced apoptosis and G0/G1 phase cell cycle arrest through caspase-3/7 cleavage. Activities of MIP2 and COX-2 were downregulated by 0.2- and 4.6-fold respectively in the HMM mushroom extract-treated ORL-204 cells. Novelty and scientific contribution: Using ORL-204, we showed that HMM mushroom extract may act via the TNF pathway at various network sites as a potential dietary compound for cancer prevention and natural adjunct therapeutic to conventional cancer treatment.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Food Technol Biotechnol Año: 2022 Tipo del documento: Article País de afiliación: Malasia Pais de publicación: Croacia
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Food Technol Biotechnol Año: 2022 Tipo del documento: Article País de afiliación: Malasia Pais de publicación: Croacia