Your browser doesn't support javascript.
loading
Separation of native and C106-oxidized DJ-1 proteins by using column chromatography.
Choi, Joonhyeok; Yoo, Hee-Jin; Cho, Kun; Kim, Hak Nam; Lee, Joon-Hwa; Ryu, Kyoung-Seok.
Afiliación
  • Choi J; Ochang Center, Korea Basic Science Institute, 162 Yeongudanji-Ro, Ochang-Eup, Chungcheongbuk-Do, 28119, South Korea.
  • Yoo HJ; Ochang Center, Korea Basic Science Institute, 162 Yeongudanji-Ro, Ochang-Eup, Chungcheongbuk-Do, 28119, South Korea; Department of Chemistry, Sogang University, 35, Baekbeom-ro, Mapo-gu, Seoul, 04107, South Korea.
  • Cho K; Ochang Center, Korea Basic Science Institute, 162 Yeongudanji-Ro, Ochang-Eup, Chungcheongbuk-Do, 28119, South Korea; Department of Bio-Analytical Science, University of Science and Technology, 217 Gajeong-Ro, Yuseong-Gu, Daejeon, 34113, South Korea.
  • Kim HN; Ochang Center, Korea Basic Science Institute, 162 Yeongudanji-Ro, Ochang-Eup, Chungcheongbuk-Do, 28119, South Korea.
  • Lee JH; Department of Chemistry and RINS, Gyeongsang National University, Gyeongnam, 52828, South Korea.
  • Ryu KS; Ochang Center, Korea Basic Science Institute, 162 Yeongudanji-Ro, Ochang-Eup, Chungcheongbuk-Do, 28119, South Korea; Department of Bio-Analytical Science, University of Science and Technology, 217 Gajeong-Ro, Yuseong-Gu, Daejeon, 34113, South Korea. Electronic address: ksryu@kbsi.re.kr.
Protein Expr Purif ; 195-196: 106092, 2022 08.
Article en En | MEDLINE | ID: mdl-35430350
Mutations in PARK7, the gene encoding the DJ-1 protein, are associated with early onset of Parkinson's disease. The C106 residue of DJ-1 is highly susceptible to oxidation, and its oxidation status is essential for various in vivo neuroprotective roles. Since C106 is readily oxidized to sulfinic acid that is not reduced by dithiothreitol, no method to separate native DJ-1 protein from the oxidized one creates challenges in the in vitro study of the biological relevance of C106-oxidation state. Here, we report an efficient column chromatography method to purify native, C106-sulfinic, and mixed (combination of the priors) forms of DJ-1. This method will be useful for systematic in vitro studies of DJ-1 functions by providing specific native and C106-sulfinic DJ-1 proteins.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedad de Parkinson / Proteínas Oncogénicas Límite: Humans Idioma: En Revista: Protein Expr Purif Asunto de la revista: BIOLOGIA MOLECULAR Año: 2022 Tipo del documento: Article País de afiliación: Corea del Sur Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedad de Parkinson / Proteínas Oncogénicas Límite: Humans Idioma: En Revista: Protein Expr Purif Asunto de la revista: BIOLOGIA MOLECULAR Año: 2022 Tipo del documento: Article País de afiliación: Corea del Sur Pais de publicación: Estados Unidos