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Poly(4-Styrenesulfonic Acid-co-maleic Anhydride)-Coated NaGdF4:Yb,Tb,Nd Nanoparticles with Luminescence and Magnetic Properties for Imaging of Pancreatic Islets and ß-Cells.
Shapoval, Oleksandr; Engstová, Hana; Jirák, Daniel; Drahokoupil, Jan; Sulková, Katerina; Berková, Zuzana; Pop-Georgievski, Ognen; Holendová, Blanka; Jezek, Petr; Horák, Daniel.
Afiliación
  • Shapoval O; Institute of Macromolecular Chemistry of the Czech Academy of Sciences, Heyrovského nám. 2, Prague 6 162 06, Czech Republic.
  • Engstová H; Institute of Physiology of the Czech Academy of Sciences, Vídenská 1083, Prague 4 142 20, Czech Republic.
  • Jirák D; Institute for Clinical and Experimental Medicine, Vídenská 1958/9, Prague 4 142 21, Czech Republic.
  • Drahokoupil J; Institute of Biophysics and Informatics, First Faculty of Medicine, Charles University, Salmovská 1, Prague 2 120 00, Czech Republic.
  • Sulková K; Institute of Physics of the Czech Academy of Sciences, Na Slovance 1999/2, Prague 8 182 21, Czech Republic.
  • Berková Z; Institute for Clinical and Experimental Medicine, Vídenská 1958/9, Prague 4 142 21, Czech Republic.
  • Pop-Georgievski O; Institute for Clinical and Experimental Medicine, Vídenská 1958/9, Prague 4 142 21, Czech Republic.
  • Holendová B; Institute of Macromolecular Chemistry of the Czech Academy of Sciences, Heyrovského nám. 2, Prague 6 162 06, Czech Republic.
  • Jezek P; Institute of Physiology of the Czech Academy of Sciences, Vídenská 1083, Prague 4 142 20, Czech Republic.
  • Horák D; Institute of Physiology of the Czech Academy of Sciences, Vídenská 1083, Prague 4 142 20, Czech Republic.
ACS Appl Mater Interfaces ; 14(16): 18233-18247, 2022 Apr 27.
Article en En | MEDLINE | ID: mdl-35416039
Novel Yb,Tb,Nd-doped GdF3 and NaGdF4 nanoparticles were synthesized by a coprecipitation method in ethylene glycol (EG) in the presence of the poly(4-styrenesulfonic acid-co-maleic anhydride) stabilizer. The particle size and morphology, crystal structure, and phase change were controlled by adjusting the PSSMA concentration and source of fluoride anions in the reaction. Doping of Yb3+, Tb3+, and Nd3+ ions in the NaGdF4 host nanoparticles induced luminescence under ultraviolet and near-infrared excitation and high relaxivity in magnetic resonance (MR) imaging (MRI). In vitro toxicity of the nanoparticles and their cellular uptake efficiency were determined in model rat pancreatic ß-cells (INS-1E). As the NaGdF4:Yb,Tb,Nd@PSSMA-EG nanoparticles were non-toxic and possessed good luminescence and magnetic properties, they were applicable for in vitro optical and MRI of isolated pancreatic islets in phantoms. The superior contrast was achieved for in vivo T2*-weighted MR images of the islets transplanted under the kidney capsule to mice in preclinical trials.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Islotes Pancreáticos / Nanopartículas Límite: Animals Idioma: En Revista: ACS Appl Mater Interfaces Asunto de la revista: BIOTECNOLOGIA / ENGENHARIA BIOMEDICA Año: 2022 Tipo del documento: Article País de afiliación: República Checa Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Islotes Pancreáticos / Nanopartículas Límite: Animals Idioma: En Revista: ACS Appl Mater Interfaces Asunto de la revista: BIOTECNOLOGIA / ENGENHARIA BIOMEDICA Año: 2022 Tipo del documento: Article País de afiliación: República Checa Pais de publicación: Estados Unidos