Your browser doesn't support javascript.
loading
Dexamethasone Attenuates the Expression of MMP-13 in Chondrocytes through MKP-1.
Lehtola, Tiina; Nummenmaa, Elina; Tuure, Lauri; Hämäläinen, Mari; Nieminen, Riina M; Moilanen, Teemu; Pemmari, Antti; Moilanen, Eeva.
Afiliación
  • Lehtola T; The Immunopharmacology Research Group, Faculty of Medicine and Health Technology, Tampere University and Tampere University Hospital, 33014 Tampere, Finland.
  • Nummenmaa E; The Immunopharmacology Research Group, Faculty of Medicine and Health Technology, Tampere University and Tampere University Hospital, 33014 Tampere, Finland.
  • Tuure L; The Immunopharmacology Research Group, Faculty of Medicine and Health Technology, Tampere University and Tampere University Hospital, 33014 Tampere, Finland.
  • Hämäläinen M; The Immunopharmacology Research Group, Faculty of Medicine and Health Technology, Tampere University and Tampere University Hospital, 33014 Tampere, Finland.
  • Nieminen RM; The Immunopharmacology Research Group, Faculty of Medicine and Health Technology, Tampere University and Tampere University Hospital, 33014 Tampere, Finland.
  • Moilanen T; The Immunopharmacology Research Group, Faculty of Medicine and Health Technology, Tampere University and Tampere University Hospital, 33014 Tampere, Finland.
  • Pemmari A; Coxa Hospital for Joint Replacement, 33520 Tampere, Finland.
  • Moilanen E; The Immunopharmacology Research Group, Faculty of Medicine and Health Technology, Tampere University and Tampere University Hospital, 33014 Tampere, Finland.
Int J Mol Sci ; 23(7)2022 Mar 31.
Article en En | MEDLINE | ID: mdl-35409238
Mitogen-activated protein kinase phosphatase-1 (MKP-1) is upregulated in inflammation and reduces the activity of proinflammatory mitogen-activated protein kinases (MAP kinases) by dephosphorylation. MAP kinases are intracellular signaling pathways that mediate the cellular effects of proinflammatory cytokines. In the present study, we investigated the effects of the glucocorticoid dexamethasone on the expression of catabolic enzymes in chondrocytes and tested the hypothesis that these effects are mediated through MKP-1. Dexamethasone was found to significantly attenuate the expression of matrix metalloproteinase (MMP)-13 in human OA chondrocytes as well as in chondrocytes from MKP-1 WT mice, but not in chondrocytes from MKP-1 KO mice. Dexamethasone also increased the expression of MKP-1 in murine and human OA chondrocytes. Furthermore, p38 MAP kinase inhibitors significantly attenuated MMP-13 expression in human OA chondrocytes, while JNK MAP kinase inhibitors had no effect. The results indicate that the effect of dexamethasone on MMP-13 expression in chondrocytes was mediated by an MKP-1 and p38 MAP kinase-dependent manner. These findings, together with previous results, support the concept of MKP-1 as a protective factor in articular chondrocytes in inflammatory conditions and as a potential drug target to treat OA.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Condrocitos / Proteínas Quinasas p38 Activadas por Mitógenos Límite: Animals Idioma: En Revista: Int J Mol Sci Año: 2022 Tipo del documento: Article País de afiliación: Finlandia Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Condrocitos / Proteínas Quinasas p38 Activadas por Mitógenos Límite: Animals Idioma: En Revista: Int J Mol Sci Año: 2022 Tipo del documento: Article País de afiliación: Finlandia Pais de publicación: Suiza