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Septin 2 interacts with dengue virus replication complex proteins and participates in virus replication in mosquito cells.
Rubio-Miranda, José Ángel; Cázares-Raga, Febe Elena; Coy-Arechavaleta, Andrea Santos; Viettri, Mercedes; Cortes-Martínez, Leticia; Lagunes-Guillén, Anel; Chávez-Munguía, Bibiana; Ludert, Juan E; Hernández-Hernández, Fidel de la Cruz.
Afiliación
  • Rubio-Miranda JÁ; Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies (CINVESTAV-IPN), Mexico City, 07360, Mexico.
  • Cázares-Raga FE; Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies (CINVESTAV-IPN), Mexico City, 07360, Mexico.
  • Coy-Arechavaleta AS; Laboratorio Central de Epidemiología, Centro Médico Nacional La Raza, Instituto Mexicano del Seguro Social, Mexico City, 02990, Mexico.
  • Viettri M; Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies (CINVESTAV-IPN), Mexico City, 07360, Mexico.
  • Cortes-Martínez L; Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies (CINVESTAV-IPN), Mexico City, 07360, Mexico.
  • Lagunes-Guillén A; Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies (CINVESTAV-IPN), Mexico City, 07360, Mexico.
  • Chávez-Munguía B; Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies (CINVESTAV-IPN), Mexico City, 07360, Mexico.
  • Ludert JE; Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies (CINVESTAV-IPN), Mexico City, 07360, Mexico. Electronic address: jludert@cinvestav.mx.
  • Hernández-Hernández FC; Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies (CINVESTAV-IPN), Mexico City, 07360, Mexico. Electronic address: cruzcruz@cinvestav.mx.
Virology ; 570: 67-80, 2022 05.
Article en En | MEDLINE | ID: mdl-35390695
Septins are a family of GTP-binding proteins identified in insects and mammals. Septins are components of the cytoskeleton and participate in cytokinesis, chromosomal segregation, intracellular vesicular traffic, and response to pathogens. Human septin 6 was identified as necessary for hepatitis C virus replication. Information about host factors necessary for flavivirus replication in mosquitoes is scarce. Thus, the role of septins in the replicative cycle of dengue virus in Aedes spp. derived cells was investigated. Through bioinformatic analysis, sequences of septin-like proteins were identified. Infected mosquito cells showed increased expression of Sep2. Colocalization analysis, proximity ligation and immunoprecipitation assays indicated that Sep2 interacts with proteins E, NS3 and NS5, but not NS1. Immunoelectron microscopy evidenced the presence of AalSep2 in replicative complexes. Finally, silencing of Sep2 expression resulted in a significant decrease in virus progeny, indicating that Sep2 is a host factor participating in dengue virus replication in mosquito cells.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Replicación Viral / Aedes / Dengue / Flavivirus Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Virology Año: 2022 Tipo del documento: Article País de afiliación: México Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Replicación Viral / Aedes / Dengue / Flavivirus Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Virology Año: 2022 Tipo del documento: Article País de afiliación: México Pais de publicación: Estados Unidos