CRISPR-Cas13a cascade-based viral RNA assay for detecting SARS-CoV-2 and its mutations in clinical samples.

Wang, Yuxi; Xue, Ting; Wang, Minjin; Ledesma-Amaro, Rodrigo; Lu, Ying; Hu, Xinyue; Zhang, Ting; Yang, Ming; Li, Yalun; Xiang, Jin; Deng, Ruijie; Ying, Binwu; Li, Weimin

Wang, Yuxi; Xue, Ting; Wang, Minjin; Ledesma-Amaro, Rodrigo; Lu, Ying; Hu, Xinyue; Zhang, Ting; Yang, Ming; Li, Yalun; Xiang, Jin; Deng, Ruijie; Ying, Binwu; Li, Weimin.

Afiliación

Wang Y; Department of Respiratory and Critical Care Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
Xue T; Targeted Tracer Research and development laboratory, Institute of Respiratory Health, Frontiers Science Center for Disease-related Molecular Network, West China Hospital, Sichuan University, Chengdu 610041, China.
Wang M; Department of Respiratory and Critical Care Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
Ledesma-Amaro R; Department of Laboratory Medicine, West China Hospital of Sichuan University, Chengdu 610041, China.
Lu Y; Department of Bioengineering, Imperial College Centre for Synthetic Biology, Imperial College London, London, UK.
Hu X; College of Biomass Science and Engineering, Sichuan University, Chengdu 610065, China.
Zhang T; State Key Laboratory of Biotherapy and Cancer Center/Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China.
Yang M; Targeted Tracer Research and development laboratory, Institute of Respiratory Health, Frontiers Science Center for Disease-related Molecular Network, West China Hospital, Sichuan University, Chengdu 610041, China.
Li Y; State Key Laboratory of Biotherapy and Cancer Center/Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China.
Xiang J; College of Biomass Science and Engineering, Sichuan University, Chengdu 610065, China.
Deng R; Department of Respiratory and Critical Care Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
Ying B; Department of Respiratory and Critical Care Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
Li W; Targeted Tracer Research and development laboratory, Institute of Respiratory Health, Frontiers Science Center for Disease-related Molecular Network, West China Hospital, Sichuan University, Chengdu 610041, China.

Sens Actuators B Chem ; 362: 131765, 2022 Jul 01.

Article en En | MEDLINE | ID: mdl-35370361

Palabras clave

COVID-19; COVID-19, coronavirus disease 2019; CRISPR, clustered regularly interspaced short palindromic repeats; CRISPR-Cas13; Cas, CRISPR associated proteins; LOD, limit of detection; NASBA, nucleic acid sequence-based amplification; Nucleic acid tests; RNA aptamer; SARS-CoV-2; SARS-CoV-2, severe acute respiratory syndrome coronavirus 2; T4 PNK, T4 Polynucleotide Kinase; crRNA, CRISPR RNA

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Sens Actuators B Chem Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza

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