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Application of microwave plasma atomic emission spectrometry in bioanalytical chemistry of bioactive rhenium compounds.
Soba, Mariano; Scalese, Gonzalo; Pérez-Díaz, Leticia; Gambino, Dinorah; Machado, Ignacio.
Afiliación
  • Soba M; Área Química Inorgánica, Facultad de Química, Universidad de la República, Montevideo, Uruguay; Área Química Analítica, Facultad de Química, Universidad de la República, Montevideo, Uruguay; Programa de Posgrado en Química, Facultad de Química, Universidad de la República, Montevideo, Uruguay.
  • Scalese G; Área Química Inorgánica, Facultad de Química, Universidad de la República, Montevideo, Uruguay.
  • Pérez-Díaz L; Laboratorio de Interacciones Moleculares, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay.
  • Gambino D; Área Química Inorgánica, Facultad de Química, Universidad de la República, Montevideo, Uruguay.
  • Machado I; Área Química Analítica, Facultad de Química, Universidad de la República, Montevideo, Uruguay. Electronic address: imachado@fq.edu.uy.
Talanta ; 244: 123413, 2022 Jul 01.
Article en En | MEDLINE | ID: mdl-35358896
Five newly synthetized fac-Re(I) tricarbonyl compounds were explored as prospective antitrypanosomal agents. The biological activity of the whole series was evaluated preliminarily against the epimastigote form of Trypanosoma cruzi. All compounds showed activity against epimastigotes with IC50 values in the low micromolar range. The most active compound [fac-Re(I)(CO)3(tmp)(CTZ)](PF6), with CTZ = clotrimazole and tmp = 3,4,7,8-tetramethyl-1,10-phenantroline, showed good selectivity towards the parasites and thus was selected to carry out further metallomic studies. For this task, a newly bioanalytical method based on microwave plasma atomic emission spectrometry (MP-AES) was developed and validated. The accuracy of the method was ensured by testing a certified reference material. Results of rhenium elemental analysis by MP-AES agreed with the proposed formula of the studied compounds, contributing to the overall validation of the method, which was then applied to evaluate the percentage of rhenium uptaken by the parasites and the association of the compounds with parasite biomacromolecules. Metallomics results showed low total rhenium percentage uptaken by parasites (∼1.2%) and preferential accumulation in the soluble proteins fraction (∼82.8%). Thus, the method based on MP-AES turned out to be an economical and green alternative for metallomics studies involving potential rhenium metallodrugs. Moreover, a comparison against rhenium determination by electrothermal atomic absorption spectrometry (ET-AAS) was included.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Renio Tipo de estudio: Observational_studies / Risk_factors_studies Idioma: En Revista: Talanta Año: 2022 Tipo del documento: Article País de afiliación: Uruguay Pais de publicación: Países Bajos
Texto completo
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Imprimir
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Renio Tipo de estudio: Observational_studies / Risk_factors_studies Idioma: En Revista: Talanta Año: 2022 Tipo del documento: Article País de afiliación: Uruguay Pais de publicación: Países Bajos