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The role of insulators and transcription in 3D chromatin organization of flies.
Chathoth, Keerthi T; Mikheeva, Liudmila A; Crevel, Gilles; Wolfe, Jareth C; Hunter, Ioni; Beckett-Doyle, Saskia; Cotterill, Sue; Dai, Hongsheng; Harrison, Andrew; Zabet, Nicolae Radu.
Afiliación
  • Chathoth KT; School of Life Sciences, University of Essex, Colchester CO4 3SQ, United Kingdom.
  • Mikheeva LA; School of Life Sciences, University of Essex, Colchester CO4 3SQ, United Kingdom.
  • Crevel G; Blizard Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London E1 2AT, United Kingdom.
  • Wolfe JC; Department of Mathematical Sciences, University of Essex, Colchester CO4 3SQ, United Kingdom.
  • Hunter I; Department Basic Medical Sciences, St. Georges University London, London SW17 0RE, United Kingdom.
  • Beckett-Doyle S; School of Life Sciences, University of Essex, Colchester CO4 3SQ, United Kingdom.
  • Cotterill S; Blizard Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London E1 2AT, United Kingdom.
  • Dai H; School of Computer Science and Electronic Engineering, University of Essex, Colchester CO4 3SQ, United Kingdom.
  • Harrison A; School of Life Sciences, University of Essex, Colchester CO4 3SQ, United Kingdom.
  • Zabet NR; School of Life Sciences, University of Essex, Colchester CO4 3SQ, United Kingdom.
Genome Res ; 32(4): 682-698, 2022 04.
Article en En | MEDLINE | ID: mdl-35354608
The DNA in many organisms, including humans, is shown to be organized in topologically associating domains (TADs). In Drosophila, several architectural proteins are enriched at TAD borders, but it is still unclear whether these proteins play a functional role in the formation and maintenance of TADs. Here, we show that depletion of BEAF-32, Cp190, Chro, and Dref leads to changes in TAD organization and chromatin loops. Their depletion predominantly affects TAD borders located in regions moderately enriched in repressive modifications and depleted in active ones, whereas TAD borders located in euchromatin are resilient to these knockdowns. Furthermore, transcriptomic data has revealed hundreds of genes displaying differential expression in these knockdowns and showed that the majority of differentially expressed genes are located within reorganized TADs. Our work identifies a novel and functional role for architectural proteins at TAD borders in Drosophila and a link between TAD reorganization and subsequent changes in gene expression.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cromatina / Proteínas de Drosophila Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Genome Res Asunto de la revista: BIOLOGIA MOLECULAR / GENETICA Año: 2022 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cromatina / Proteínas de Drosophila Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Genome Res Asunto de la revista: BIOLOGIA MOLECULAR / GENETICA Año: 2022 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Estados Unidos