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A New Human Platelet Lysate for Mesenchymal Stem Cell Production Compliant with Good Manufacturing Practice Conditions.
Mareschi, Katia; Marini, Elena; Niclot, Alessia Giovanna Santa Banche; Barone, Marta; Pinnetta, Giuseppe; Adamini, Aloe; Spadea, Manuela; Labanca, Luciana; Lucania, Graziella; Ferrero, Ivana; Fagioli, Franca.
Afiliación
  • Mareschi K; Department of Public Health and Paediatrics, The University of Turin, Piazza Polonia 94, 10126 Torino, Italy.
  • Marini E; Stem Cell Transplantation and Cellular Therapy Laboratory, Paediatric Onco-Haematology Division, Regina Margherita Children's Hospital, City of Health and Science of Turin, 10126 Torino, Italy.
  • Niclot AGSB; Department of Public Health and Paediatrics, The University of Turin, Piazza Polonia 94, 10126 Torino, Italy.
  • Barone M; Department of Public Health and Paediatrics, The University of Turin, Piazza Polonia 94, 10126 Torino, Italy.
  • Pinnetta G; Department of Public Health and Paediatrics, The University of Turin, Piazza Polonia 94, 10126 Torino, Italy.
  • Adamini A; Stem Cell Transplantation and Cellular Therapy Laboratory, Paediatric Onco-Haematology Division, Regina Margherita Children's Hospital, City of Health and Science of Turin, 10126 Torino, Italy.
  • Spadea M; Stem Cell Transplantation and Cellular Therapy Laboratory, Paediatric Onco-Haematology Division, Regina Margherita Children's Hospital, City of Health and Science of Turin, 10126 Torino, Italy.
  • Labanca L; Department of Public Health and Paediatrics, The University of Turin, Piazza Polonia 94, 10126 Torino, Italy.
  • Lucania G; Blood Component Production and Validation Center, City of Health and Science of Turin, S. Anna Hospital, 10126 Turin, Italy.
  • Ferrero I; Blood Component Production and Validation Center, City of Health and Science of Turin, S. Anna Hospital, 10126 Turin, Italy.
  • Fagioli F; Stem Cell Transplantation and Cellular Therapy Laboratory, Paediatric Onco-Haematology Division, Regina Margherita Children's Hospital, City of Health and Science of Turin, 10126 Torino, Italy.
Int J Mol Sci ; 23(6)2022 Mar 17.
Article en En | MEDLINE | ID: mdl-35328655
Mesenchymal stem cells (MSCs) are classified as advanced therapy medicinal products, a new category of GMP (good manufacturing practice)-compliant medicines for clinical use. We isolated MSCs from 5 bone marrow (BM) samples using human platelet lysate (HPL) instead of foetal bovine serum (FBS). We used a new method of HPL production consisting of treating platelet (PLTs) pools with Ca-Gluconate to form a gel clot, then mechanically squeezing to release growth factors. We compared the new HPL (HPL-S) with the standard (HPL-E) obtained by freezing/thawing cycles and by adding heparin. HPL-S had not PLTs and fibrinogen but the quantity of proteins and growth factors was comparable to HPL-E. Therefore, HPL-S needed fewer production steps to be in compliance with GMP conditions. The number of colonies forming unit-fibroblasts (CFU-F) and the maintenance of stem markers showed no significant differences between MSCs with HPL-E and HPL-S. The cumulative population doubling was higher in MSCs with HPL-E in the earlier passages, but we observed an inverted trend of cell growth at the fourth passage. Immunophenotypic analysis showed a significant lower expression of HLA-DR in the MSCs with HPL-S (1.30%) than HPL-E (14.10%). In conclusion, we demonstrated that HPL-S is an effective alternative for MSC production under GMP conditions.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Madre Mesenquimatosas Límite: Humans Idioma: En Revista: Int J Mol Sci Año: 2022 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Madre Mesenquimatosas Límite: Humans Idioma: En Revista: Int J Mol Sci Año: 2022 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Suiza