Your browser doesn't support javascript.
loading
Optimizing detection of erythropoietin receptor agonists from dried blood spots for anti-doping application.
Heiland, Carmel E; Ericsson, Magnus; Pohanka, Anton; Ekström, Lena; Marchand, Alexandre.
Afiliación
  • Heiland CE; Department of Laboratory Medicine, Karolinska Institute, Stockholm, Sweden.
  • Ericsson M; Clinical Pharmacology, Karolinska University Laboratory, Karolinska University Hospital, Stockholm, Sweden.
  • Pohanka A; Department of Laboratory Medicine, Karolinska Institute, Stockholm, Sweden.
  • Ekström L; Laboratoire AntiDopage Français (LADF), University of Paris-Saclay, Châtenay-Malabry, France.
  • Marchand A; Department of Laboratory Medicine, Karolinska Institute, Stockholm, Sweden.
Drug Test Anal ; 14(8): 1377-1386, 2022 Aug.
Article en En | MEDLINE | ID: mdl-35322582
The World Anti-Doping Agency (WADA) has recently implemented dried blood spots (DBSs) as a matrix for doping control. However, specifications regarding the analysis of the class of prohibited substances called erythropoietin (EPO) receptor agonists (ERAs) from DBSs are not yet described. The aim of this study was to find optimal conditions (sample volume and storage) to sensitively detect endogenous erythropoietin (hEPO) and prohibited ERAs from DBSs and compare detection limits to WADA-stipulated minimum required performance levels (MRPLs) for ERAs in serum/plasma samples. Venous whole blood was spotted onto Whatman 903 DBS cards with primarily 60 µl of blood, but various volumes from 20 to75 µl were tested. All samples were immunopurified with MAIIA EPO Purification Gel kit (EPGK) and analysed with sodium N-lauroylsarcosinate polyacrylamide gel electrophoresis (SAR-PAGE) and Western blot. Sixty-microliter DBSs allowed the detection of the four main ERAs (BRP, NESP, CERA and EPO-Fc) at concentrations close to WADA's MRPLs described for 500 µl of serum/plasma. Different storage temperatures, from -20°C to 37°C, were evaluated and did not affect ERA detection. A comparison of the detection of endogenous EPO from the different anti-doping matrices (urine, serum and DBSs produced from upper arm capillary blood) from five participants for 6 weeks was performed. Endogenous EPO extracted from DBSs showed intra-individual variations in male and female subjects, but less than in urine. Doping controls would benefit from the stability of ERAs on DBSs: It can be a complementary matrix for ERA analysis, particularly in the absence of EPO signals in urine.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Detección de Abuso de Sustancias / Receptores de Eritropoyetina / Doping en los Deportes / Hematínicos Tipo de estudio: Diagnostic_studies Límite: Female / Humans / Male Idioma: En Revista: Drug Test Anal Asunto de la revista: FARMACOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Detección de Abuso de Sustancias / Receptores de Eritropoyetina / Doping en los Deportes / Hematínicos Tipo de estudio: Diagnostic_studies Límite: Female / Humans / Male Idioma: En Revista: Drug Test Anal Asunto de la revista: FARMACOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Reino Unido