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Structural insight into African swine fever virus I73R protein reveals it as a Z-DNA binding protein.
Sun, Lifang; Miao, Yurun; Wang, Zhenzhong; Chen, Huan; Dong, Panpan; Zhang, Hong; Wu, Linjiao; Jiang, Meiqin; Chen, Lifei; Yang, Wendi; Lin, Pingdong; Jing, Dingding; Luo, Zhipu; Zhang, Yongqiang; Jung, Yong-Sam; Wu, Xiaodong; Qian, Yingjuan; Wu, Yunkun.
Afiliación
  • Sun L; Provincial University Key Laboratory of Cellular Stress Response and Metabolic Regulation, College of Life Science, Fujian Normal University, Fuzhou, China.
  • Miao Y; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu, China.
  • Wang Z; Jiangsu Foreign Expert Workshop, Nanjing, Jiangsu, China.
  • Chen H; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu, China.
  • Dong P; China Animal Health and Epidemiology Center, Qingdao, China.
  • Zhang H; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu, China.
  • Wu L; Jiangsu Foreign Expert Workshop, Nanjing, Jiangsu, China.
  • Jiang M; Provincial University Key Laboratory of Cellular Stress Response and Metabolic Regulation, College of Life Science, Fujian Normal University, Fuzhou, China.
  • Chen L; Provincial University Key Laboratory of Cellular Stress Response and Metabolic Regulation, College of Life Science, Fujian Normal University, Fuzhou, China.
  • Yang W; Provincial University Key Laboratory of Cellular Stress Response and Metabolic Regulation, College of Life Science, Fujian Normal University, Fuzhou, China.
  • Lin P; Provincial University Key Laboratory of Cellular Stress Response and Metabolic Regulation, College of Life Science, Fujian Normal University, Fuzhou, China.
  • Jing D; Provincial University Key Laboratory of Cellular Stress Response and Metabolic Regulation, College of Life Science, Fujian Normal University, Fuzhou, China.
  • Luo Z; Provincial University Key Laboratory of Cellular Stress Response and Metabolic Regulation, College of Life Science, Fujian Normal University, Fuzhou, China.
  • Zhang Y; Provincial University Key Laboratory of Cellular Stress Response and Metabolic Regulation, College of Life Science, Fujian Normal University, Fuzhou, China.
  • Jung YS; Provincial University Key Laboratory of Cellular Stress Response and Metabolic Regulation, College of Life Science, Fujian Normal University, Fuzhou, China.
  • Wu X; School of Biology and Basic Medical Sciences, Institute of Molecular Enzymology, Soochow University, Suzhou, Jiangsu, China.
  • Qian Y; China Animal Health and Epidemiology Center, Qingdao, China.
  • Wu Y; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu, China.
Transbound Emerg Dis ; 69(5): e1923-e1935, 2022 Sep.
Article en En | MEDLINE | ID: mdl-35312168
African Swine Fever (ASF) is a highly contagious viral haemorrhagic disease of swine, leading to enormous economic losses in the swine industry. However, vaccines and drugs to treat ASF have yet to be developed. African swine fever virus (ASFV) encodes more than 150 proteins, but 50% of them have unknown functions. Here, we present the crystal structure of the ASFV I73R protein at a resolution of 2.0 Å. Similar search tools based solely on amino acid sequence shows that it has no relationships to any proteins of known function. Interestingly, the overall structure of the I73R protein shares a winged helix-turn-helix fold, structural similarity with the Z-DNA binding domain (Zα). In accordance with this result, the I73R is capable of binding to a CpG repeats DNA duplex, which has a high propensity for forming Z-DNA during the DNA binding assays. In addition, the I73R protein was shown to be expressed at both early and late stages of ASFV post-infection in PAM cells as an 8.9 kDa protein. Immunofluorescence studies revealed that the I73R protein is expressed in the nucleus at early times post-infection and gradually translocated from the nucleus to the cytoplasm. Taken together, these data indicate that the I73R could be a member of Zα family that is important in host-pathogen interaction, which paves the way for the design of inhibitors to target this severe pathogen. Further exploring the biological role of I73R during ASFV infection in vitro and in vivo will provide new clues for development of new antiviral strategies.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de los Porcinos / Fiebre Porcina Africana / Virus de la Fiebre Porcina Africana / ADN de Forma Z Límite: Animals Idioma: En Revista: Transbound Emerg Dis Asunto de la revista: MEDICINA VETERINARIA Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de los Porcinos / Fiebre Porcina Africana / Virus de la Fiebre Porcina Africana / ADN de Forma Z Límite: Animals Idioma: En Revista: Transbound Emerg Dis Asunto de la revista: MEDICINA VETERINARIA Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Alemania