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Molecular detection of mycobacterium tuberculosis complex species in triatomines found in urban households and Peridomiciles in a city with high prevalence of tuberculosis in Northeastern Brazil.
Silva, Caroliny Soares; Gondim Costa Lima, Luana Nepomuceno; Batista Lima, Karla Val Ria; Freitas de Almeida, Rosa Livia; da Conceição, MarIlia Lima; Parente, Cynara Carvalho; Rocha, Amanda Albuquerque; da Silva, Thales Candido; Sancho, Soraya de Oliveira; Frota, Cristiane Cunha.
Afiliación
  • Silva CS; Department of Pathology and Legal Medicine, Federal University of Ceará, Fortaleza, Brazil.
  • Gondim Costa Lima LN; Bacteriology and Mycology Section, Evandro Chagas Institute, Ananindeua; Postgraduate Program in Parasitic Biology in the Amazon, State University of Pará, Belém, Brazil.
  • Batista Lima KVR; Bacteriology and Mycology Section, Evandro Chagas Institute, Ananindeua; Postgraduate Program in Parasitic Biology in the Amazon, State University of Pará, Belém, Brazil.
  • Freitas de Almeida RL; Postgraduate Program in Public Health, Fortaleza University, Fortaleza, Brazil.
  • da Conceição ML; Bacteriology and Mycology Section, Evandro Chagas Institute, Ananindeua, Brazil.
  • Parente CC; Medicine School, Federal University of Ceará Sobral, Brazil.
  • Rocha AA; Sobral Zoonoses Surveillance Unit, Sobral Health Secretary, Sobral, Brazil.
  • da Silva TC; Department of Pathology and Legal Medicine, Federal University of Ceará, Fortaleza, Brazil.
  • Sancho SO; Department of Pathology and Legal Medicine, Federal University of Ceará, Fortaleza, Brazil.
  • Frota CC; Department of Pathology and Legal Medicine, Federal University of Ceará, Fortaleza, Brazil.
Int J Mycobacteriol ; 11(1): 51-59, 2022.
Article en En | MEDLINE | ID: mdl-35295024
Background: Triatomine insects, native to northeastern Brazil, have been found in the urban peridomicile. The city of Sobral has a high number of tuberculosis cases and several triatomine species. This study investigates the presence of mycobacteria, particularly Mycobacterium tuberculosis complex (MTBC) species, in triatomines captured in the urban perimeter of Sobral. Methods: We analyzed 167 triatomines captured in urban households and peridomiciles of Sobral. Mycobacteria were identified by the PRA-hsp65 method followed by partial sequencing of the hsp65 and rpoB genes. The sequences confirmed as MTBC were also typed by mycobacterial interspersed repetitive units-variable number tandem repeats (MIRU-VNTR) and spoligotyping. Results: Triatoma brasiliensis (38.6%), Triatoma pseudomaculata (32.9%), Panstrongylus lutzi (24.3%) were the most frequently identified. In 51.1% (70/167) of them, species of the Mycobacteriaceae family were detected by PRA-hsp65; of these, 31.4% (22/70) were identified as belonging to MTBC species. Nine (12.9%) of the triatomine samples were confirmed by sequencing as belonging to MTBC species. MIRU-VNTR genotyping suggests that the presence of different MTBC sublines in the triatomines should be investigated. Conclusion: This is the first report of MTBC lineages in triatomine insects. These results indicate the migration and adaptation of these insects in an urban setting.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tuberculosis / Mycobacterium tuberculosis Tipo de estudio: Diagnostic_studies / Prevalence_studies / Prognostic_studies / Risk_factors_studies Límite: Humans País/Región como asunto: America do sul / Brasil Idioma: En Revista: Int J Mycobacteriol Año: 2022 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: India
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tuberculosis / Mycobacterium tuberculosis Tipo de estudio: Diagnostic_studies / Prevalence_studies / Prognostic_studies / Risk_factors_studies Límite: Humans País/Región como asunto: America do sul / Brasil Idioma: En Revista: Int J Mycobacteriol Año: 2022 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: India