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A Cell Membrane Targeting Molybdenum-Iodine Nanocluster: Rational Ligand Design toward Enhanced Photodynamic Activity.
Kirakci, Kaplan; Kubánová, Michaela; Pribyl, Tomás; Rumlová, Michaela; Zelenka, Jaroslav; Ruml, Tomás; Lang, Kamil.
Afiliación
  • Kirakci K; Institute of Inorganic Chemistry of the Czech Academy of Sciences, Rez 1001, 250 68 Husinec-Rez, Czech Republic.
  • Kubánová M; Department of Biochemistry and Microbiology, University of Chemistry and Technology Prague, 166 28 Praha 6, Czech Republic.
  • Pribyl T; Department of Biochemistry and Microbiology, University of Chemistry and Technology Prague, 166 28 Praha 6, Czech Republic.
  • Rumlová M; Department of Biotechnology, University of Chemistry and Technology Prague, 166 28 Praha, Czech Republic.
  • Zelenka J; Department of Biochemistry and Microbiology, University of Chemistry and Technology Prague, 166 28 Praha 6, Czech Republic.
  • Ruml T; Department of Biochemistry and Microbiology, University of Chemistry and Technology Prague, 166 28 Praha 6, Czech Republic.
  • Lang K; Institute of Inorganic Chemistry of the Czech Academy of Sciences, Rez 1001, 250 68 Husinec-Rez, Czech Republic.
Inorg Chem ; 61(12): 5076-5083, 2022 Mar 28.
Article en En | MEDLINE | ID: mdl-35293732
The development of singlet oxygen photosensitizers, which target specific cellular organelles, constitutes a pertinent endeavor to optimize the efficiency of photodynamic therapy. Targeting of the cell membrane eliminates the need for endocytosis of drugs that can lead to toxicity, intracellular degradation, or drug resistance. In this context, we utilized copper-free click chemistry to prepare a singlet oxygen photosensitizing complex, made of a molybdenum-iodine nanocluster stabilized by triazolate apical ligands. In phosphate-buffered saline, the complex formed nanoaggregates with a positive surface charge due to the protonatable amine function of the apical ligands. These nanoaggregates targeted cell membranes and caused an eminent blue-light phototoxic effect against HeLa cells at nanomolar concentrations, inducing apoptotic cell death, while having no dark toxicity at physiologically relevant concentrations. The properties of this complex were compared to those of a negatively charged parent complex to highlight the dominant effect of the nature of apical ligands on biological properties of the nanocluster. These two complexes also exerted (photo)antibacterial effects on several pathogenic strains in the form of planktonic cultures and biofilms. Overall, we demonstrated that the rational design of apical ligands toward cell membrane targeting leads to enhanced photodynamic efficiency.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Yodo / Molibdeno Límite: Humans Idioma: En Revista: Inorg Chem Año: 2022 Tipo del documento: Article País de afiliación: República Checa Pais de publicación: Estados Unidos
Texto completo
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XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Yodo / Molibdeno Límite: Humans Idioma: En Revista: Inorg Chem Año: 2022 Tipo del documento: Article País de afiliación: República Checa Pais de publicación: Estados Unidos