ALB, albumin; ASGR2, asialoglycoprotein receptor 2; ATP1a1, ATPases subunit alpha-1; BMP4, bone morphogenetic protein 4; BSA-FAF, bovine serum albumin fatty acid-free; BSEP, bile salt export pump; Bile acid transport; CDFDA, 5-(and-6)-carboxy-2',7'-dichlorofluorescein; Cellular polarity; DE, definitive endoderm; DILI, drug-induced liver injury; FGF2, fibroblast growth factor 2; GCA, glycocholate; GCDCA, glycochenodeoxycholate; HCM, Hepatocyte Culture Medium; HE, hepatic endodermal; HGF, hepatocyte growth factor; HNF4a, hepatic nuclear factor 4a; MDCKII, Madin­Darby canine kidney II; MRP2, multidrug resistance-associated protein 2; NTCP, Na+-TCA cotransporter; PFIC (progressive familial intrahepatic cholestasis); PFIC, progressive familial intrahepatic cholestasis; PI, propidium iodide; RT-qPCR, quantitative reverse transcription PCR; TCA, taurocholic acid; TCDCA, taurochenodeoxycholate; TEER, transepithelial electrical resistance; TEM, transmission electron microscopy; TJP1, tight junction protein 1; TJP2, tight junction protein 2; iHep, iPSC-derived hepatocytes; iPSC, induced pluripotent stem cell; sgRNA, single-guide RNA; ssODN, single-stranded oligonucleotide-DNA