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Overexpression of FcεRI on Bone Marrow Mast Cells, but Not MRGPRX2, in Clonal Mast Cell Disorders With Wasp Venom Anaphylaxis.
Elst, Jessy; De Puysseleyr, Leander P; Ebo, Didier G; Faber, Margaretha A; Van Gasse, Athina L; van der Poorten, Marie-Line M; Decuyper, Ine I; Bridts, Chris H; Mertens, Christel; Van Houdt, Michel; Hagendorens, Margo M; De Clerck, Luc S; Verlinden, Anke; Vermeulen, Katrien; Maes, Marie-Berthe; Berneman, Zwi N; Valent, Peter; Sabato, Vito.
Afiliación
  • Elst J; Department of Immunology, Allergology, Rheumatology and the Infla-Med Centre of Excellence, Faculty of Medicine and Health Sciences, University of Antwerp, Antwerp, Belgium.
  • De Puysseleyr LP; Immunology, Allergology, Rheumatology, Antwerp University Hospital, Antwerp, Belgium.
  • Ebo DG; Department of Immunology, Allergology, Rheumatology and the Infla-Med Centre of Excellence, Faculty of Medicine and Health Sciences, University of Antwerp, Antwerp, Belgium.
  • Faber MA; Immunology, Allergology, Rheumatology, Antwerp University Hospital, Antwerp, Belgium.
  • Van Gasse AL; Department of Immunology, Allergology, Rheumatology and the Infla-Med Centre of Excellence, Faculty of Medicine and Health Sciences, University of Antwerp, Antwerp, Belgium.
  • van der Poorten MM; Immunology, Allergology, Rheumatology, Antwerp University Hospital, Antwerp, Belgium.
  • Decuyper II; Department of Immunology and Allergology, AZ Jan Palfijn Gent, Ghent, Belgium.
  • Bridts CH; Department of Immunology, Allergology, Rheumatology and the Infla-Med Centre of Excellence, Faculty of Medicine and Health Sciences, University of Antwerp, Antwerp, Belgium.
  • Mertens C; Immunology, Allergology, Rheumatology, Antwerp University Hospital, Antwerp, Belgium.
  • Van Houdt M; Department of Immunology, Allergology, Rheumatology and the Infla-Med Centre of Excellence, Faculty of Medicine and Health Sciences, University of Antwerp, Antwerp, Belgium.
  • Hagendorens MM; Immunology, Allergology, Rheumatology, Antwerp University Hospital, Antwerp, Belgium.
  • De Clerck LS; Department of Paediatrics and the Infla-Med Centre of Excellence, Faculty of Medicine and Health Sciences, University of Antwerp, Antwerp, Belgium.
  • Verlinden A; Paediatrics, Antwerp University Hospital, Antwerp, Belgium.
  • Vermeulen K; Department of Immunology, Allergology, Rheumatology and the Infla-Med Centre of Excellence, Faculty of Medicine and Health Sciences, University of Antwerp, Antwerp, Belgium.
  • Maes MB; Immunology, Allergology, Rheumatology, Antwerp University Hospital, Antwerp, Belgium.
  • Berneman ZN; Department of Paediatrics and the Infla-Med Centre of Excellence, Faculty of Medicine and Health Sciences, University of Antwerp, Antwerp, Belgium.
  • Valent P; Paediatrics, Antwerp University Hospital, Antwerp, Belgium.
  • Sabato V; Department of Immunology, Allergology, Rheumatology and the Infla-Med Centre of Excellence, Faculty of Medicine and Health Sciences, University of Antwerp, Antwerp, Belgium.
Front Immunol ; 13: 835618, 2022.
Article en En | MEDLINE | ID: mdl-35281031
Background: Uncertainties remain about the molecular mechanisms governing clonal mast cell disorders (CMCD) and anaphylaxis. Objective: This study aims at comparing the burden, phenotype and behavior of mast cells (MCs) and basophils in patients with CMCD with wasp venom anaphylaxis (CMCD/WVA+), CMCD patients without anaphylaxis (CMCD/ANA-), patients with an elevated baseline serum tryptase (EBST), patients with wasp venom anaphylaxis without CMCD (WVA+) and patients with a non-mast cell haematological pathology (NMHP). Methods: This study included 20 patients with CMCD/WVA+, 24 with CMCD/ANA-, 19 with WVA+, 6 with EBST and 5 with NMHP. We immunophenotyped MCs and basophils and compared baseline serum tryptase (bST) and both total and venom specific IgE in the different groups. For basophil studies, 13 healthy controls were also included. Results: Higher levels of bST were found in CMCD patients with wasp venom anaphylaxis, CMCD patients without anaphylaxis and EBST patients. Total IgE levels were highest in patients with wasp venom anaphylaxis with and without CMCD. Bone marrow MCs of patients with CMCD showed lower CD117 expression and higher expression of CD45, CD203c, CD63, CD300a and FcεRI. Within the CMCD population, patients with wasp venom anaphylaxis showed a higher expression of FcεRI as compared to patients without anaphylaxis. Expression of MRGPRX2 on MCs did not differ between the study populations. Basophils are phenotypically and functionally comparable between the different patient populations. Conclusion: Patients with CMCD show an elevated burden of aberrant activated MCs with a significant overexpression of FcεRI in patients with a wasp venom anaphylaxis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Mastocitosis / Anafilaxia Límite: Humans Idioma: En Revista: Front Immunol Año: 2022 Tipo del documento: Article País de afiliación: Bélgica Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Mastocitosis / Anafilaxia Límite: Humans Idioma: En Revista: Front Immunol Año: 2022 Tipo del documento: Article País de afiliación: Bélgica Pais de publicación: Suiza