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In vitro evaluation of the cis-[Ru(phen)2(pPDIp)]2+⁎⁎ complex for antimicrobial photodynamic therapy against Sporothrix brasiliensis and Candida albicans.
Tiburcio, M A; Rocha, A R; Romano, R A; Inada, N M; Bagnato, V S; Carlos, R M; Buzzá, H H.
Afiliación
  • Tiburcio MA; Chemistry Department, Federal University of São Carlos, Brazil. Electronic address: tiburcioamarco@gmail.com.
  • Rocha AR; São Carlos Institute of Physics, University of Sao Paulo, Brazil; PPG Biotec, Federal University of São Carlos, Brazil.
  • Romano RA; São Carlos Institute of Physics, University of Sao Paulo, Brazil.
  • Inada NM; São Carlos Institute of Physics, University of Sao Paulo, Brazil.
  • Bagnato VS; São Carlos Institute of Physics, University of Sao Paulo, Brazil; Hagler Fellow, Texas A&M University, College Station, TX, USA.
  • Carlos RM; Chemistry Department, Federal University of São Carlos, Brazil.
  • Buzzá HH; São Carlos Institute of Physics, University of Sao Paulo, Brazil; Institute of Physics, Pontificia Universidad Católica de Chile, Santiago, Chile. Electronic address: hilde.buzza@uc.cl.
J Photochem Photobiol B ; 229: 112414, 2022 Apr.
Article en En | MEDLINE | ID: mdl-35276578
BACKGROUND: Photodynamic therapy (PDT) activates a photosensitizer by visible light to generate cytotoxic oxygen species that lead to cell death. With proper illumination, PDT is often used in applications on superficial and sub-surface lesions. Sporotrichosis infection occurs by Sporothrix fungi which causes a skin wound, worsened by Candida albicans infections. This study investigated the photosensitizing efficiency of the Ru(phen)2(pPDIp)(PF6)2 complex, RupPDIp, against S. brasiliensis and C. albicans. MATERIAL AND METHODS: RupPDIp efficiency against these fungi was tested using 450 nm (blue light and 36 J/cm2) and 525 nm (green light, 25.2 J/cm2) at 0.05-20 µM concentrations. To ensure PDT effectiveness, control groups were tested in the absence and in the presence of RupPDIp under light irradiation and in the dark. RESULTS: RupPDIp eliminated both fungi at ≤5.0 µM. Green light showed the best results, eliminating S. brasiliensis and C. albicans colonies at RupPDIp 0.5 µM and 0.05 µM, respectively. CONCLUSION: RupPDIp is a promising photosensitizer in aPDT, eliminating 106 CFU/mL of both fungi at 450 nm and 525 nm, with lower light doses and concentrations when treated with the green light compared to the blue light.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fotoquimioterapia / Sporothrix / Antiinfecciosos País/Región como asunto: America do sul / Brasil Idioma: En Revista: J Photochem Photobiol B Asunto de la revista: BIOLOGIA Año: 2022 Tipo del documento: Article Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fotoquimioterapia / Sporothrix / Antiinfecciosos País/Región como asunto: America do sul / Brasil Idioma: En Revista: J Photochem Photobiol B Asunto de la revista: BIOLOGIA Año: 2022 Tipo del documento: Article Pais de publicación: Suiza