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GATA4/5/6 family transcription factors are conserved determinants of cardiac versus pharyngeal mesoderm fate.
Song, Mengyi; Yuan, Xuefei; Racioppi, Claudia; Leslie, Meaghan; Stutt, Nathan; Aleksandrova, Anastasiia; Christiaen, Lionel; Wilson, Michael D; Scott, Ian C.
Afiliación
  • Song M; Program in Developmental and Stem Cell Biology, The Hospital for Sick Children, Toronto, Canada.
  • Yuan X; Program in Genetics and Genome Biology, The Hospital for Sick Children, Toronto, Canada.
  • Racioppi C; Department of Molecular Genetics, University of Toronto, Toronto, Canada.
  • Leslie M; Program in Developmental and Stem Cell Biology, The Hospital for Sick Children, Toronto, Canada.
  • Stutt N; Program in Genetics and Genome Biology, The Hospital for Sick Children, Toronto, Canada.
  • Aleksandrova A; Center for Developmental Genetics, Department of Biology, New York University, New York, NY, USA.
  • Christiaen L; Program in Developmental and Stem Cell Biology, The Hospital for Sick Children, Toronto, Canada.
  • Wilson MD; Department of Molecular Genetics, University of Toronto, Toronto, Canada.
  • Scott IC; Program in Developmental and Stem Cell Biology, The Hospital for Sick Children, Toronto, Canada.
Sci Adv ; 8(10): eabg0834, 2022 03 11.
Article en En | MEDLINE | ID: mdl-35275720
GATA4/5/6 transcription factors play essential, conserved roles in heart development. To understand how GATA4/5/6 modulates the mesoderm-to-cardiac fate transition, we labeled, isolated, and performed single-cell gene expression analysis on cells that express gata5 at precardiac time points spanning zebrafish gastrulation to somitogenesis. We found that most mesendoderm-derived lineages had dynamic gata5/6 expression. In the absence of Gata5/6, the population structure of mesendoderm-derived cells was substantially altered. In addition to the expected absence of cardiac mesoderm, we confirmed a concomitant expansion of cranial-pharyngeal mesoderm. Moreover, Gata5/6 loss led to extensive changes in chromatin accessibility near cardiac and pharyngeal genes. Functional analyses in zebrafish and the tunicate Ciona, which has a single GATA4/5/6 homolog, revealed that GATA4/5/6 acts upstream of tbx1 to exert essential and cell-autonomous roles in promoting cardiac and inhibiting pharyngeal mesoderm identity. Overall, cardiac and pharyngeal mesoderm fate choices are achieved through an evolutionarily conserved GATA4/5/6 regulatory network.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pez Cebra / Factor de Transcripción GATA4 Límite: Animals Idioma: En Revista: Sci Adv Año: 2022 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pez Cebra / Factor de Transcripción GATA4 Límite: Animals Idioma: En Revista: Sci Adv Año: 2022 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Estados Unidos