Your browser doesn't support javascript.
loading
Approach to map nanotopography of cell surface receptors.
Franke, Christian; Chum, Tomás; Kvícalová, Zuzana; Glatzová, Daniela; Gentsch, Gregor Jörg; Rodriguez, Alvaro; Helmerich, Dominic A; Herdly, Lucas; Mavila, Harsha; Frank, Otakar; Brdicka, Tomás; van de Linde, Sebastian; Cebecauer, Marek.
Afiliación
  • Franke C; Institute of Applied Optics and Biophysics, Friedrich Schiller University Jena, Jena, Germany. christian.franke@uni-jena.de.
  • Chum T; Jena Center for Soft Matter, Friedrich Schiller University Jena, Jena, Germany. christian.franke@uni-jena.de.
  • Kvícalová Z; Abbe Center of Photonics, Friedrich Schiller University Jena, Jena, Germany. christian.franke@uni-jena.de.
  • Glatzová D; Department of Biophysical Chemistry, J. Heyrovsky Institute of Physical Chemistry, Czech Academy of Sciences, Prague, Czech Republic.
  • Gentsch GJ; Department of Biophysical Chemistry, J. Heyrovsky Institute of Physical Chemistry, Czech Academy of Sciences, Prague, Czech Republic.
  • Rodriguez A; Department of Biophysical Chemistry, J. Heyrovsky Institute of Physical Chemistry, Czech Academy of Sciences, Prague, Czech Republic.
  • Helmerich DA; Laboratory of Leukocyte Signalling, Institute of Molecular Genetics, Czech Academy of Sciences, Prague, Czech Republic.
  • Herdly L; Institute of Applied Optics and Biophysics, Friedrich Schiller University Jena, Jena, Germany.
  • Mavila H; Department of Electrochemical Materials, J. Heyrovsky Institute of Physical Chemistry, Czech Academy of Sciences, Prague, Czech Republic.
  • Frank O; Department of Biotechnology and Biophysics, Biocenter, University of Würzburg, Würzburg, Germany.
  • Brdicka T; Department of Physics, SUPA, University of Strathclyde, Glasgow, UK.
  • van de Linde S; Department of Biophysical Chemistry, J. Heyrovsky Institute of Physical Chemistry, Czech Academy of Sciences, Prague, Czech Republic.
  • Cebecauer M; Department of Electrochemical Materials, J. Heyrovsky Institute of Physical Chemistry, Czech Academy of Sciences, Prague, Czech Republic.
Commun Biol ; 5(1): 218, 2022 03 09.
Article en En | MEDLINE | ID: mdl-35264712
Cells communicate with their environment via surface receptors, but nanoscopic receptor organization with respect to complex cell surface morphology remains unclear. This is mainly due to a lack of accessible, robust and high-resolution methods. Here, we present an approach for mapping the topography of receptors at the cell surface with nanometer precision. The method involves coating glass coverslips with glycine, which preserves the fine membrane morphology while allowing immobilized cells to be positioned close to the optical surface. We developed an advanced and simplified algorithm for the analysis of single-molecule localization data acquired in a biplane detection scheme. These advancements enable direct and quantitative mapping of protein distribution on ruffled plasma membranes with near isotropic 3D nanometer resolution. As demonstrated successfully for CD4 and CD45 receptors, the described workflow is a straightforward quantitative technique to study molecules and their interactions at the complex surface nanomorphology of differentiated metazoan cells.
Asunto(s)
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Receptores de Superficie Celular / Nanotecnología Límite: Animals Idioma: En Revista: Commun Biol Año: 2022 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Receptores de Superficie Celular / Nanotecnología Límite: Animals Idioma: En Revista: Commun Biol Año: 2022 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Reino Unido