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Anti-inflammatory and anti-necrotic effects of lectins from Canavalia ensiformis and Canavalia brasiliensis in experimental acute pancreatitis.
Oliveira, Samara Rodrigues Bonfim Damasceno; Franco, Álvaro Xavier; Quaresma, Marielle Pires; de Carvalho, Cecília Mendes Morais; da Cunha Jácome Marques, Fabrícia; da Silva Pantoja, Patrícia; Mendonça, Vanessa Azevedo; da Silva Osterne, Vinicius José; Correia, Jorge Luis Almeida; Assreuy, Ana Maria Sampaio; de Souza, Marcellus Henrique Loiola Pontes; do Nascimento, Kyria Santiago; Cavada, Benildo Sousa; Criddle, David Neil; Soares, Pedro Marcos Gomes.
Afiliación
  • Oliveira SRBD; Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, Ceará, Brazil.
  • Franco ÁX; Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, Ceará, Brazil.
  • Quaresma MP; Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, Ceará, Brazil.
  • de Carvalho CMM; Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, Ceará, Brazil.
  • da Cunha Jácome Marques F; Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, Ceará, Brazil.
  • da Silva Pantoja P; Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, Ceará, Brazil.
  • Mendonça VA; Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, Ceará, Brazil.
  • da Silva Osterne VJ; Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, Ceará, Brazil.
  • Correia JLA; Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, Ceará, Brazil.
  • Assreuy AMS; Institute of Biomedical Sciences, State University of Ceará, Fortaleza, Ceará, Brazil.
  • de Souza MHLP; Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, Ceará, Brazil.
  • do Nascimento KS; Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, Ceará, Brazil.
  • Cavada BS; Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, Ceará, Brazil.
  • Criddle DN; Department of Molecular Physiology & Cell Signalling, ISMIB, University of Liverpool, Liverpool, UK.
  • Soares PMG; Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, Ceará, Brazil. pedrogsoares@yahoo.com.br.
Glycoconj J ; 39(5): 599-608, 2022 10.
Article en En | MEDLINE | ID: mdl-35239112
Lectins isolated from Canavalia ensiformis (ConA) and Canavalia brasiliensis (ConBr) are promising molecules to prevent cell death. Acute pancreatitis, characterized by acinar cell necrosis and inflammation, presents significant morbidity and mortality. This study has investigated the effects of ConA and ConBr in experimental acute pancreatitis and pancreatic acinar cell death induced by bile acid. Pancreatitis was induced by retrograde pancreatic ductal injection of 3% sodium taurocholate (Na-TC) in male Swiss mice. ConA or ConBr (0.1, 1 or 10 mg/kg) were intravenously applied to mice 1 h and 12 h after induction. After 24 h, the severity of pancreatitis was evaluated by serum amylase and lipase, histopathological changes and myeloperoxidase assay. Pancreatic acinar cells were incubated with ConA (200 µg/ml) or ConBr (200 µg/ml) and taurolithocholic acid 3-sulfate (TLCS; 500 µM). Necrosis and changes in mitochondrial membrane potential (ΔÑ°m) were detected by fluorescence confocal microscopy. Treatment (post-insult) with ConA and ConBr decreased pancreatic damage caused by retrograde injection of Na-TC in mice, reducing pancreatic neutrophil infiltration, edema and necrosis. In addition, ConA and ConBr decreased pancreatic acinar cell necrosis and depolarization of ΔÑ°m caused by TLCS. The inhibition of necrosis was prevented by the lectin domain blockade. In conclusion, ConA and ConBr markedly inhibited in vitro and in vivo damage, effects partly dependent on the interaction with mannose residues on acinar cells. These data support the potential application of these proteins for treatment of acute pancreatitis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pancreatitis / Canavalia Límite: Animals País/Región como asunto: America do sul / Brasil Idioma: En Revista: Glycoconj J Asunto de la revista: BIOQUIMICA / METABOLISMO Año: 2022 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pancreatitis / Canavalia Límite: Animals País/Región como asunto: America do sul / Brasil Idioma: En Revista: Glycoconj J Asunto de la revista: BIOQUIMICA / METABOLISMO Año: 2022 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos