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Synthesis and characterization of a collagen-based composite material containing selenium nanoparticles.
Stevanovic, Magdalena M; Filipovic, Nenad; Kuzmanovic, Maja; Tomic, Nina; Usjak, Dusan; Milenkovic, Marina; Zheng, Kai; Stampfl, Juergen; Boccaccini, Aldo R.
Afiliación
  • Stevanovic MM; 201271Institute of Technical Sciences of the Serbian Academy of Sciences and Arts, Beograd, Serbia.
  • Filipovic N; 201271Institute of Technical Sciences of the Serbian Academy of Sciences and Arts, Beograd, Serbia.
  • Kuzmanovic M; 201271Institute of Technical Sciences of the Serbian Academy of Sciences and Arts, Beograd, Serbia.
  • Tomic N; 201271Institute of Technical Sciences of the Serbian Academy of Sciences and Arts, Beograd, Serbia.
  • Usjak D; Department of Microbiology and Immunology, 186111University of Belgrade Faculty of Pharmacy, Beograd, Serbia.
  • Milenkovic M; Department of Microbiology and Immunology, 186111University of Belgrade Faculty of Pharmacy, Beograd, Serbia.
  • Zheng K; Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, Erlangen, Germany.
  • Stampfl J; Institute of Materials Science and Technology, TU Wien, Wien, Austria.
  • Boccaccini AR; Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, Erlangen, Germany.
J Biomater Appl ; 36(10): 1800-1811, 2022 05.
Article en En | MEDLINE | ID: mdl-35225050
Multidrug-resistant bacterial strains represent an emerging global health threat and a great obstacle for bone tissue engineering. One of the major components of the extracellular matrix of the bone is a collagen protein, while selenium is an element that has antimicrobial potential, and is also important for bone metabolism and bone health. Here we represent the incorporation of selenium nanoparticles (SeNPs) synthesized by the green chemical reduction method into collagen gels to produce a composite material, collagen/SeNPs, with antimicrobial properties. The samples were comprehensively characterized by zeta potential measurements, dynamic light scattering inductively coupled plasma-mass spectrometry (ICP-MS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), optical microscopy, field-emission scanning electron microscopy (FE-SEM), and differential scanning calorimetry The cytotoxicity of the SeNPS, as well as collagen/SeNPs, was tested on the MRC-5 cells. It was revealed that collagen/SeNPS expressed a lower cytotoxic effect. Collagen/SeNPs showed significant antibacterial activity against all tested Gram-positive strains, the major causative agents of orthopedic infections as well as Candida albicans. Furthermore, three-dimensional ß-tricalcium phosphate (3D-TCP) scaffolds were fabricated by a well-established 3D printing (lithography) method, and afterward preliminary coated by newly-synthesized SeNPs or collagen/SeNPs. In addition, uncoated 3D-TCP scaffolds as well as coated by collagen/SeNPs were subjected to biofilm formation. The production of Staphylococcus aureus biofilm on coated scaffolds by collagen/SeNPs was significantly reduced compared to the uncoated ones.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Selenio / Nanopartículas Idioma: En Revista: J Biomater Appl Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2022 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Selenio / Nanopartículas Idioma: En Revista: J Biomater Appl Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2022 Tipo del documento: Article Pais de publicación: Reino Unido