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Collection of Monoclonal Antibodies Targeting SARS-CoV-2 Proteins.
Pribanic Matesic, Marina; Kucan Brlic, Paola; Lenac Rovis, Tihana; Macak Safranko, Zeljka; Chaouat, Abigael Eva; Miklic, Karmela; Malic, Suzana; Ivankovic, Nina; Schubert, Maren; Bertoglio, Federico; Markotic, Alemka; Mandelboim, Ofer; Jonjic, Stipan; Brizic, Ilija.
Afiliación
  • Pribanic Matesic M; Center for Proteomics, Faculty of Medicine, University of Rijeka, Brace Branchetta 20, 51000 Rijeka, Croatia.
  • Kucan Brlic P; Center for Proteomics, Faculty of Medicine, University of Rijeka, Brace Branchetta 20, 51000 Rijeka, Croatia.
  • Lenac Rovis T; Center for Proteomics, Faculty of Medicine, University of Rijeka, Brace Branchetta 20, 51000 Rijeka, Croatia.
  • Macak Safranko Z; Research Department, University Hospital for Infectious Diseases "Dr. Fran Mihaljevic", Mirogojska 8, 10000 Zagreb, Croatia.
  • Chaouat AE; The Concern Foundation Laboratories at the Lautenberg Center for Immunology and Cancer Research, The Hebrew University Hadassah Medical School, Jerusalem 9112001, Israel.
  • Miklic K; Center for Proteomics, Faculty of Medicine, University of Rijeka, Brace Branchetta 20, 51000 Rijeka, Croatia.
  • Malic S; Center for Proteomics, Faculty of Medicine, University of Rijeka, Brace Branchetta 20, 51000 Rijeka, Croatia.
  • Ivankovic N; Research Department, University Hospital for Infectious Diseases "Dr. Fran Mihaljevic", Mirogojska 8, 10000 Zagreb, Croatia.
  • Schubert M; Institut für Biochemie, Biotechnologie und Bioinformatik, Abteilung Biotechnologie, Technische Universität Braunschweig, Spielmannstr. 7, 38106 Braunschweig, Germany.
  • Bertoglio F; Institut für Biochemie, Biotechnologie und Bioinformatik, Abteilung Biotechnologie, Technische Universität Braunschweig, Spielmannstr. 7, 38106 Braunschweig, Germany.
  • Markotic A; Research Department, University Hospital for Infectious Diseases "Dr. Fran Mihaljevic", Mirogojska 8, 10000 Zagreb, Croatia.
  • Mandelboim O; The Concern Foundation Laboratories at the Lautenberg Center for Immunology and Cancer Research, The Hebrew University Hadassah Medical School, Jerusalem 9112001, Israel.
  • Jonjic S; Center for Proteomics, Faculty of Medicine, University of Rijeka, Brace Branchetta 20, 51000 Rijeka, Croatia.
  • Brizic I; Center for Proteomics, Faculty of Medicine, University of Rijeka, Brace Branchetta 20, 51000 Rijeka, Croatia.
Viruses ; 14(2)2022 02 21.
Article en En | MEDLINE | ID: mdl-35216036
In early 2020, the COVID-19 pandemic sparked a global crisis that continues to pose a serious threat to human health and the economy. Further advancement in research is necessary and requires the availability of quality molecular tools, including monoclonal antibodies. Here, we present the development and characterization of a collection of over 40 new monoclonal antibodies directed against different SARS-CoV-2 proteins. Recombinant SARS-CoV-2 proteins were expressed, purified, and used as immunogens. Upon development of specific hybridomas, the obtained monoclonal antibody (mAb) clones were tested for binding to recombinant proteins and infected cells. We generated mAbs against structural proteins, the Spike and Nucleocapsid protein, several non-structural proteins (nsp1, nsp7, nsp8, nsp9, nsp10, nsp16) and accessory factors (ORF3a, ORF9b) applicable in flow cytometry, immunofluorescence, or Western blot. Our collection of mAbs provides a set of novel, highly specific tools that will allow a comprehensive analysis of the viral proteome, which will allow further understanding of SARS-CoV-2 pathogenesis and the design of therapeutic strategies.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Virales / SARS-CoV-2 / Anticuerpos Monoclonales / Anticuerpos Antivirales Límite: Humans Idioma: En Revista: Viruses Año: 2022 Tipo del documento: Article País de afiliación: Croacia Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Virales / SARS-CoV-2 / Anticuerpos Monoclonales / Anticuerpos Antivirales Límite: Humans Idioma: En Revista: Viruses Año: 2022 Tipo del documento: Article País de afiliación: Croacia Pais de publicación: Suiza