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Salmon Erythrocytes Sequester Active Virus Particles in Infectious Salmon Anaemia.
Fosse, Johanna Hol; Aamelfot, Maria; Sønstevold, Tonje; Weli, Simon Chioma; Vendramin, Niccolò; Petersen, Petra Elisabeth; Solhaug, Anita; Amundsen, Marit Måsøy; Heffernan, Inger Austrheim; Cuenca, Argelia; Christiansen, Debes Hammershaimb; Falk, Knut.
Afiliación
  • Fosse JH; Norwegian Veterinary Institute, 1433 Ås, Norway.
  • Aamelfot M; Norwegian Veterinary Institute, 1433 Ås, Norway.
  • Sønstevold T; Norwegian Veterinary Institute, 1433 Ås, Norway.
  • Weli SC; Norwegian Veterinary Institute, 1433 Ås, Norway.
  • Vendramin N; Unit for Fish and Shellfish Diseases, National Institute of Aquatic Resources, Technical University of Denmark, 2800 Kongens Lyngby, Denmark.
  • Petersen PE; National Reference Laboratory for Fish and Animal Diseases, Faroese Food and Veterinary Authority, 110 Tórshavn, Faroe Islands.
  • Solhaug A; Norwegian Veterinary Institute, 1433 Ås, Norway.
  • Amundsen MM; Norwegian Veterinary Institute, 1433 Ås, Norway.
  • Heffernan IA; Norwegian Veterinary Institute, 1433 Ås, Norway.
  • Cuenca A; Unit for Fish and Shellfish Diseases, National Institute of Aquatic Resources, Technical University of Denmark, 2800 Kongens Lyngby, Denmark.
  • Christiansen DH; National Reference Laboratory for Fish and Animal Diseases, Faroese Food and Veterinary Authority, 110 Tórshavn, Faroe Islands.
  • Falk K; Norwegian Veterinary Institute, 1433 Ås, Norway.
Viruses ; 14(2)2022 02 02.
Article en En | MEDLINE | ID: mdl-35215905
Infectious salmon anaemia virus (ISAV) binds circulating Atlantic salmon erythrocytes, but the relevance of this interaction for the course of infection and development of disease remains unclear. We here characterise ISAV-erythrocyte interactions in experimentally infected Atlantic salmon and show that ISAV-binding to erythrocytes is common and precedes the development of disease. Viral RNA and infective particles were enriched in the cellular fraction of blood. While erythrocyte-associated ISAV remained infectious, erythrocytes dose-dependently limited the infection of cultured cells. Surprisingly, immunostaining of blood smears revealed expression of ISAV proteins in a small fraction of erythrocytes in one of the examined trials, confirming that ISAV can be internalised in this cell type and engage the cellular machinery in transcription and translation. However, viral protein expression in erythrocytes was rare and not required for development of disease and mortality. Furthermore, active transcription of ISAV mRNA was higher in tissues than in blood, supporting the assumption that ISAV replication predominantly takes place in endothelial cells. In conclusion, Atlantic salmon erythrocytes bind ISAV and sequester infective virus particles during infection, but do not appear to significantly contribute to ISAV replication. We discuss the implications of our findings for infection dynamics and pathogenesis of infectious salmon anaemia.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones por Orthomyxoviridae / Salmo salar / Isavirus / Eritrocitos / Enfermedades de los Peces Límite: Animals Idioma: En Revista: Viruses Año: 2022 Tipo del documento: Article País de afiliación: Noruega Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones por Orthomyxoviridae / Salmo salar / Isavirus / Eritrocitos / Enfermedades de los Peces Límite: Animals Idioma: En Revista: Viruses Año: 2022 Tipo del documento: Article País de afiliación: Noruega Pais de publicación: Suiza