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Comparison of three different protocols for obtaining hemolysis.
Nikolac Gabaj, Nora; Miler, Marijana; Vrtaric, Alen; Celap, Ivana; Bocan, Marina; Filipi, Petra; Radisic Biljak, Vanja; Simundic, Ana-Maria; Supak Smolcic, Vesna; Kocijancic, Marija.
Afiliación
  • Nikolac Gabaj N; Working Group for Preanalytical Phase of the Croatian Society of Medical Biochemistry and Laboratory Medicine, Zagreb, Croatia.
  • Miler M; Department of Clinical Chemistry, Sestre milosrdnice University Hospital Center, Zagreb, Croatia.
  • Vrtaric A; Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatia.
  • Celap I; Working Group for Preanalytical Phase of the Croatian Society of Medical Biochemistry and Laboratory Medicine, Zagreb, Croatia.
  • Bocan M; Department of Clinical Chemistry, Sestre milosrdnice University Hospital Center, Zagreb, Croatia.
  • Filipi P; Working Group for Preanalytical Phase of the Croatian Society of Medical Biochemistry and Laboratory Medicine, Zagreb, Croatia.
  • Radisic Biljak V; Department of Clinical Chemistry, Sestre milosrdnice University Hospital Center, Zagreb, Croatia.
  • Simundic AM; Working Group for Preanalytical Phase of the Croatian Society of Medical Biochemistry and Laboratory Medicine, Zagreb, Croatia.
  • Supak Smolcic V; Department of Clinical Chemistry, Sestre milosrdnice University Hospital Center, Zagreb, Croatia.
  • Kocijancic M; Working Group for Preanalytical Phase of the Croatian Society of Medical Biochemistry and Laboratory Medicine, Zagreb, Croatia.
Clin Chem Lab Med ; 60(5): 714-725, 2022 04 26.
Article en En | MEDLINE | ID: mdl-35212494
OBJECTIVES: Hemolysis is associated with erroneous or delayed results. Objectives of the study were to compare four different methods for obtaining hemolysis in vitro on three different analyzers. METHODS: Hemolysis was prepared with addition of pure hemoglobin into serum pool, osmotic shock, aspiration through blood collection needle, freezing/thawing of whole blood. Biochemistry parameters were measured in duplicate at Architect c8000 (Abbott, Abbott Park, USA), Beckman Coulter AU680 (Beckman Coulter, Brea, USA) and Cobas 6000 c501 (Roche, Mannheim, Germany), according to manufacturers' declarations. Cut-off value was defined as the highest value of H index with corresponding bias lower than acceptance criteria. RESULTS: We were not able to obtain results with freezing protocol. On all three platforms, lowest number of analytes were sensitive to hemolysis at H=0.5 using method of adding free hemoglobin. When osmotic shock was used, cut-off values for the most analytes were generally met at lower values. Hemolysis significantly interfered with measurement of potassium and lactate dehydrogenase (LD) at H=0.5 on all platforms. The most of the tested analytes had the lowest acceptable H index when aspiration method was used. At the low level of hemolysis (H=0.8) glucose, sodium, potassium, chloride, phosphate, and LD were affected on all analyzers, with some additional analytes depending on the manufacturer. CONCLUSIONS: Hemolysis interference differs on different analyzers and according to protocol for obtaining hemolysis. Aspiration method was generally the most sensitive to hemolysis interference, while addition of free Hb was the most resistant.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sodio / Hemólisis Límite: Humans Idioma: En Revista: Clin Chem Lab Med Asunto de la revista: QUIMICA CLINICA / TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2022 Tipo del documento: Article País de afiliación: Croacia Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sodio / Hemólisis Límite: Humans Idioma: En Revista: Clin Chem Lab Med Asunto de la revista: QUIMICA CLINICA / TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2022 Tipo del documento: Article País de afiliación: Croacia Pais de publicación: Alemania