Your browser doesn't support javascript.
loading
Rapid Multiplex Strip Test for the Detection of Circulating Tumor DNA Mutations for Liquid Biopsy Applications.
Kalligosfyri, Panagiota M; Nikou, Sofia; Karteri, Sofia; Kalofonos, Haralabos P; Bravou, Vasiliki; Kalogianni, Despina P.
Afiliación
  • Kalligosfyri PM; Department of Chemistry, University of Patras, Rio, 26504 Patras, Greece.
  • Nikou S; Department of Anatomy-Histology-Embryology, Medical School, University of Patras, Rio, 26504 Patras, Greece.
  • Karteri S; Division of Oncology, Department of Internal Medicine, University Hospital of Patras, Rio, 26504 Patras, Greece.
  • Kalofonos HP; Division of Oncology, Department of Internal Medicine, University Hospital of Patras, Rio, 26504 Patras, Greece.
  • Bravou V; Department of Anatomy-Histology-Embryology, Medical School, University of Patras, Rio, 26504 Patras, Greece.
  • Kalogianni DP; Department of Chemistry, University of Patras, Rio, 26504 Patras, Greece.
Biosensors (Basel) ; 12(2)2022 Feb 04.
Article en En | MEDLINE | ID: mdl-35200357
In the era of personalized medicine, molecular profiling of patient tumors has become the standard practice, especially for patients with advanced disease. Activating point mutations of the KRAS proto-oncogene are clinically relevant for many types of cancer, including colorectal cancer (CRC). While several approaches have been developed for tumor genotyping, liquid biopsy has been gaining much attention in the clinical setting. Analysis of circulating tumor DNA for genetic alterations has been challenging, and many methodologies with both advantages and disadvantages have been developed. We here developed a gold nanoparticle-based rapid strip test that has been applied for the first time for the multiplex detection of KRAS mutations in circulating tumor DNA (ctDNA) of CRC patients. The method involved ctDNA isolation, PCR-amplification of the KRAS gene, multiplex primer extension (PEXT) reaction, and detection with a multiplex strip test. We have optimized the efficiency and specificity of the multiplex strip test in synthetic DNA targets, in colorectal cancer cell lines, in tissue samples, and in blood-derived ctDNA from patients with advanced colorectal cancer. The proposed strip test achieved rapid and easy multiplex detection (normal allele and three major single-point mutations) of the clinically relevant KRAS mutations in ctDNA in blood samples of CRC patients with high specificity and repeatability. This multiplex strip test represents a minimally invasive, rapid, low-cost, and promising diagnostic tool for the detection of clinically relevant mutations in cancer patients.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Colorrectales / Nanopartículas del Metal / ADN Tumoral Circulante Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Biosensors (Basel) Año: 2022 Tipo del documento: Article País de afiliación: Grecia Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Colorrectales / Nanopartículas del Metal / ADN Tumoral Circulante Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Biosensors (Basel) Año: 2022 Tipo del documento: Article País de afiliación: Grecia Pais de publicación: Suiza