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lncRNA FGD5-AS1 Regulates Bone Marrow Stem Cell Proliferation and Apoptosis by Affecting miR-296-5p/STAT3 Axis in Steroid-Induced Osteonecrosis of the Femoral Head.
Wu, Yadi; Fang, Lun; Gao, Yong; Zhao, Zhiqiu; Zhou, Lu; Zhang, Gang.
Afiliación
  • Wu Y; Laboratory, Shandong First Medical University, Shandong Academy of Medical Sciences, Tai'an 271016, Shandong Province, China.
  • Fang L; Laboratory, Shandong First Medical University, Shandong Academy of Medical Sciences, Tai'an 271016, Shandong Province, China.
  • Gao Y; Department of Orthopedics, The Second Affiliated Hospital of Shandong First Medical University, Tai'an 271000, Shandong Province, China.
  • Zhao Z; Laboratory, Shandong First Medical University, Shandong Academy of Medical Sciences, Tai'an 271016, Shandong Province, China.
  • Zhou L; Institute of Sports Medicine, Shandong First Medical University, Shandong Academy of Medical Sciences, Tai'an 271016, Shandong Province, China.
  • Zhang G; Department of Orthopedics, The Second Affiliated Hospital of Shandong First Medical University, Tai'an 271000, Shandong Province, China.
J Healthc Eng ; 2022: 9364467, 2022.
Article en En | MEDLINE | ID: mdl-35190765
BACKGROUND: Osteonecrosis of the femoral head (ONFH) is a common hip joint disease, which is more harmful and seriously affects the lives of patients. This study aims to clarify the regulatory mechanism of lncRNA FGD5-AS1 in ONFH. METHODS: The expression of the protein and mRNA was detected by RT-qPCR and Western blot assay. The regulatory mechanism of lncRNA FGD5-AS1 was detected by the dual-luciferase reporter assay, CCK-8 assay, and flow cytometry assay. RESULTS: Dex can inhibit cell proliferation and differentiation and induce apoptosis in hBMSCs in a dose-dependent manner. Overexpression of lncRNA FGD5-AS1 promoted cell proliferation and restrained apoptosis in Dex-treated hBMSCs. In addition, lncRNA FGD5-AS1 acts as a sponge for miR-296-5p. Also, miR-296-5p directly targets STAT3. More importantly, miR-296-5p and STAT3 can affect the function of lncRNA FGD5-AS1 in Dex-treated hBMSCs. CONCLUSION: lncRNA FGD5-AS1 promotes cell proliferation and inhibits apoptosis in steroid-induced ONFH through acting as a sponge for miR-296-5p and upregulation of STAT3.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteonecrosis / MicroARNs / ARN Largo no Codificante Límite: Humans Idioma: En Revista: J Healthc Eng Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
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Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteonecrosis / MicroARNs / ARN Largo no Codificante Límite: Humans Idioma: En Revista: J Healthc Eng Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido