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Diagnostic Potential of Plasma Extracellular Vesicle miR-483-3p and Let-7d-3p for Sepsis.
Qiu, Guanguan; Fan, Jiajie; Zheng, Guoping; He, Jiangping; Lin, Fangping; Ge, Menghua; Huang, Lanfang; Wang, Jiangmei; Xia, Jie; Huang, Ruoqiong; Shu, Qiang; Xu, Jianguo.
Afiliación
  • Qiu G; Shaoxing Second Hospital, Shaoxing, China.
  • Fan J; Department of Thoracic and Cardiovascular Surgery, Children's Hospital of Zhejiang University School of Medicine and National Clinical Research Center for Child Health, Hangzhou, China.
  • Zheng G; Shaoxing Second Hospital, Shaoxing, China.
  • He J; Shaoxing Second Hospital, Shaoxing, China.
  • Lin F; Shaoxing Second Hospital, Shaoxing, China.
  • Ge M; Shaoxing Second Hospital, Shaoxing, China.
  • Huang L; Shaoxing Second Hospital, Shaoxing, China.
  • Wang J; Department of Thoracic and Cardiovascular Surgery, Children's Hospital of Zhejiang University School of Medicine and National Clinical Research Center for Child Health, Hangzhou, China.
  • Xia J; Department of Thoracic and Cardiovascular Surgery, Children's Hospital of Zhejiang University School of Medicine and National Clinical Research Center for Child Health, Hangzhou, China.
  • Huang R; Department of Thoracic and Cardiovascular Surgery, Children's Hospital of Zhejiang University School of Medicine and National Clinical Research Center for Child Health, Hangzhou, China.
  • Shu Q; Department of Thoracic and Cardiovascular Surgery, Children's Hospital of Zhejiang University School of Medicine and National Clinical Research Center for Child Health, Hangzhou, China.
  • Xu J; Department of Thoracic and Cardiovascular Surgery, Children's Hospital of Zhejiang University School of Medicine and National Clinical Research Center for Child Health, Hangzhou, China.
Front Mol Biosci ; 9: 814240, 2022.
Article en En | MEDLINE | ID: mdl-35187084
Background: microRNAs (miRNAs) from circulating extracellular vesicles (EVs) have been reported as disease biomarkers. This study aimed to identify the diagnostic value of plasma EV-miRNAs in sepsis. Methods: EVs were separated from the plasma of sepsis patients at admission and healthy controls. The expression of EV-miRNAs was evaluated by microarray and qRT-PCR. Results: A preliminary miRNA microarray of plasma EVs from a discovery cohort of 3 sepsis patients at admission and three healthy controls identified 11 miRNAs with over 2-fold upregulation in sepsis group. Based on this finding, EV samples from a validation cohort of 37 sepsis patients at admission and 25 healthy controls were evaluated for the expression of the 6 miRNAs relating injury and inflammation via qRT-PCR. Elevated expression of miR-483-3p and let-7d-3p was validated in sepsis patients and corroborated in a mouse model of sepsis. miR-483-3p and let-7d-3p levels positively correlated with the disease severity. Additionally, a combination of miR-483-3p and let-7d-3p had diagnostic value for sepsis. Furthermore, bioinformatic analysis and experimental validation showed that miR-483-3p and let-7d-3p target pathways regulating immune response and endothelial function. Conclusion: The present study reveals the potential role of plasma EV-miRNAs in the pathogenesis of sepsis and the utility of combining miR-483-3p and let-7d-3p as biomarkers for early sepsis diagnosis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies / Prognostic_studies Idioma: En Revista: Front Mol Biosci Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies / Prognostic_studies Idioma: En Revista: Front Mol Biosci Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza