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Multicolor light-sheet microscopy for a large field of view imaging: A comparative study between Bessel and Gaussian light-sheets configurations.
Luna-Palacios, Yryx Y; Licea-Rodriguez, Jacob; Camacho-Lopez, M Dolores; Teichert, Ines; Riquelme, Meritxell; Rocha-Mendoza, Israel.
Afiliación
  • Luna-Palacios YY; Department of Optics, Centro de Investigación Científica y de Educación Superior de Ensenada, CICESE, Carretera Ensenada-Tijuana, Ensenada, Mexico.
  • Licea-Rodriguez J; Department of Optics, Centro de Investigación Científica y de Educación Superior de Ensenada, CICESE, Carretera Ensenada-Tijuana, Ensenada, Mexico.
  • Camacho-Lopez MD; Department of Microbiology, Centro de Investigación Científica y de Educación Superior de Ensenada, CICESE, Carretera Ensenada-Tijuana, Ensenada, Mexico.
  • Teichert I; Cátedras CONACYT-Deparment of Optics, Centro de Investigación Científica y de Educación Superior de Ensenada, Ensenada, Mexico.
  • Riquelme M; Department of General and Molecular Botany, Ruhr-University Bochum, Bochum, Germany.
  • Rocha-Mendoza I; Department of Microbiology, Centro de Investigación Científica y de Educación Superior de Ensenada, CICESE, Carretera Ensenada-Tijuana, Ensenada, Mexico.
J Biophotonics ; 15(6): e202100359, 2022 06.
Article en En | MEDLINE | ID: mdl-35184408
Light-sheet fluorescence microscopy (LSFM) is useful for developmental biology studies, which require a simultaneous visualization of dynamic microstructures over large fields of views (FOVs). A comparative study between multicolor Bessel and Gaussian-based LSFM systems is presented. Discussing the chromatic implications to achieve colocalized and large FOVs when both optical arrays are implemented under the same excitation objective is the purpose of this work. The light-sheets FOVs, optical sectioning, and resolution are experimentally characterized and discussed. The advantages of using Bessel beams and the main drawbacks of using Gaussian beams for multicolor imaging are highlighted. Multiple Bessel excitation minimizes the FOV's mismatch's effects due to the beams chromatic defocusing and alleviates the aside object blurring obtained with multiple Gaussian beams. It also offers a fair homogeneous axial resolution and optical sectioning over a larger effective FOV. Imaging over perithecia samples of the fungus Sordaria macrospora demonstrates such advantages. This work complements previous comparative studies that discuss only single wavelengths light-sheets excitations.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Histológicas Idioma: En Revista: J Biophotonics Asunto de la revista: BIOFISICA Año: 2022 Tipo del documento: Article País de afiliación: México Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Histológicas Idioma: En Revista: J Biophotonics Asunto de la revista: BIOFISICA Año: 2022 Tipo del documento: Article País de afiliación: México Pais de publicación: Alemania