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Non contrast enhanced volumetric histology of blood clots through high resolution propagation-based X-ray microtomography.
Saghamanesh, Somayeh; Dumitriu LaGrange, Daniela; Reymond, Philippe; Wanke, Isabel; Lövblad, Karl-Olof; Neels, Antonia; Zboray, Robert.
Afiliación
  • Saghamanesh S; Center for X-Ray Analytics, Swiss Federal Laboratories for Materials Science and Technology (Empa), 8600, Dübendorf, Switzerland. somayeh.saghamanesh@empa.ch.
  • Dumitriu LaGrange D; Neuroradiology Division, Diagnostic Department, University of Geneva, Geneva, Switzerland.
  • Reymond P; Neuroradiology Division, Diagnostic Department, University of Geneva, Geneva, Switzerland.
  • Wanke I; Neuroradiology Division, Klinik Hirslanden, Zurich, Switzerland.
  • Lövblad KO; Neuroradiology Division, Diagnostic Department, University of Geneva, Geneva, Switzerland.
  • Neels A; Faculty of Medicine, University of Geneva, Geneva, Switzerland.
  • Zboray R; Center for X-Ray Analytics, Swiss Federal Laboratories for Materials Science and Technology (Empa), 8600, Dübendorf, Switzerland.
Sci Rep ; 12(1): 2778, 2022 02 17.
Article en En | MEDLINE | ID: mdl-35177767
We have demonstrated the capability of laboratory propagation-based microtomography (miroCT) in non-destructive 3D virtual histopathology of human blood clots without any contrast agent. The volumetric information are valuable to understand the mechanical properties of clots which are crucial in selecting the most efficient mechanical thrombectomy method for clot extraction. Different clot types retrieved by mechanical thrombectomy from patient victims of acute ischemic stroke were evaluated through propagation-based microCT. The results were correlated with high-resolution scanning electron microscopy (SEM) images, confirming detected cellular and fibrillary structures. Calcifications appeared as glassy opacity areas with relatively intense signal on microCT images, also proved by energy-dispersive spectroscopy and X-ray diffraction. Hyperintense regions on the microCT corresponded to individual or compact aggregates of red blood cells, whereas fibrin dominated volumes appeared at consistently moderate to low normalized microCT values. Red blood cell shapes and sizes are consistent with the SEM observations. Together with other potential parameters, 3D porosity distribution and volume fraction of structures can be easily measured by microCT data. Further development of automated post-processing techniques for X-ray propagation-based micro/nanoCT, also based on machine learning algorithms, can enable high throughput analysis of blood clot composition and their 3D histological features on large sample cohorts.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trombosis / Microtomografía por Rayos X Límite: Humans Idioma: En Revista: Sci Rep Año: 2022 Tipo del documento: Article País de afiliación: Suiza Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trombosis / Microtomografía por Rayos X Límite: Humans Idioma: En Revista: Sci Rep Año: 2022 Tipo del documento: Article País de afiliación: Suiza Pais de publicación: Reino Unido