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Potential role of inducible GPR3 expression under stimulated T cell conditions.
Shiraki, Hiroko; Tanaka, Shigeru; Guo, Yun; Harada, Kana; Hide, Izumi; Yasuda, Tomoharu; Sakai, Norio.
Afiliación
  • Shiraki H; Department of Molecular and Pharmacological Neuroscience, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan.
  • Tanaka S; Department of Molecular and Pharmacological Neuroscience, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan. Electronic address: tanakamd@hiroshima-u.ac.jp.
  • Guo Y; Department of Immunology, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan.
  • Harada K; Department of Molecular and Pharmacological Neuroscience, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan.
  • Hide I; Department of Molecular and Pharmacological Neuroscience, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan.
  • Yasuda T; Department of Immunology, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan.
  • Sakai N; Department of Molecular and Pharmacological Neuroscience, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan.
J Pharmacol Sci ; 148(3): 307-314, 2022 Mar.
Article en En | MEDLINE | ID: mdl-35177210
G protein-coupled receptor 3 (GPR3) constitutively activates Gαs proteins without any ligands and is predominantly expressed in neurons. Since the expression and physiological role of GPR3 in immune cells is still unknown, we examined the possible role of GPR3 in T lymphocytes. The expression of GPR3 was upregulated 2 h after phorbol 12-myristate 13-acetate (PMA)/ionomycin stimulation and was sustained in Jurkat cells, a human T lymphocyte cell line. In addition, the expression of nuclear receptor 4 group A member 2 (NR4A2) was highly modulated by GPR3 expression. Additionally, GPR3 expression was linked with the transcriptional promoter activity of NR4A in Jurkat cells. In mouse CD4+ T cells, transient GPR3 expression was induced immediately after the antigen receptor stimulation. However, the expression of NR4A2 was not modulated in CD4+ T cells from GPR3-knockout mice after stimulation, and the population of Treg cells in thymocytes and splenocytes was not affected by GPR3 knockout. By contrast, spontaneous effector activation in both CD4+ T cells and CD8+ T cells was observed in GPR3-knockout mice. In summary, GPR3 is immediately induced by T cell stimulation and play an important role in the suppression of effector T cell activation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Activación de Linfocitos / Linfocitos T / Receptores Acoplados a Proteínas G Límite: Animals Idioma: En Revista: J Pharmacol Sci Asunto de la revista: FARMACOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Japón
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Activación de Linfocitos / Linfocitos T / Receptores Acoplados a Proteínas G Límite: Animals Idioma: En Revista: J Pharmacol Sci Asunto de la revista: FARMACOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Japón