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Berberine-loaded liquid crystalline nanoparticles inhibit non-small cell lung cancer proliferation and migration in vitro.
Paudel, Keshav R; Mehta, Meenu; Yin, Geena Hew Suet; Yen, Lee Li; Malyla, Vamshikrishna; Patel, Vyoma K; Panneerselvam, Jithendra; Madheswaran, Thiagarajan; MacLoughlin, Ronan; Jha, Niraj Kumar; Gupta, Piyush Kumar; Singh, Sachin Kumar; Gupta, Gaurav; Kumar, Pradeep; Oliver, Brian G; Hansbro, Philip M; Chellappan, Dinesh Kumar; Dua, Kamal.
Afiliación
  • Paudel KR; School of Life Sciences, University of Technology Sydney, Sydney, NSW, 2007, Australia.
  • Mehta M; Centre for Inflammation, Centenary Institute, Sydney, NSW, 2050, Australia.
  • Yin GHS; Centre for Inflammation, Centenary Institute, Sydney, NSW, 2050, Australia.
  • Yen LL; Discipline of Pharmacy, Graduate School of Health, University of Technology Sydney, Sydney, NSW, 2007, Australia.
  • Malyla V; School of Pharmacy, International Medical University, Bukit Jalil 57000, Kuala Lumpur, Malaysia.
  • Patel VK; School of Pharmacy, International Medical University, Bukit Jalil 57000, Kuala Lumpur, Malaysia.
  • Panneerselvam J; Centre for Inflammation, Centenary Institute, Sydney, NSW, 2050, Australia.
  • Madheswaran T; Discipline of Pharmacy, Graduate School of Health, University of Technology Sydney, Sydney, NSW, 2007, Australia.
  • MacLoughlin R; School of Life Sciences, University of Technology Sydney, Sydney, NSW, 2007, Australia.
  • Jha NK; Centre for Inflammation, Centenary Institute, Sydney, NSW, 2050, Australia.
  • Gupta PK; Department of Pharmaceutical Technology, School of Pharmacy, International Medical University, Bukit Jalil 57000, Kuala Lumpur, Malaysia.
  • Singh SK; Department of Pharmaceutical Technology, School of Pharmacy, International Medical University, Bukit Jalil 57000, Kuala Lumpur, Malaysia.
  • Gupta G; IDA Business Park, Dangan, H91 HE94, Galway, Ireland.
  • Kumar P; School of Pharmacy & Biomolecular Sciences, Royal College of Surgeons in Ireland, Dublin, D02 YN77, Ireland.
  • Oliver BG; School of Pharmacy & Pharmaceutical Sciences, Trinity College, Dublin, D02 PN40, Ireland.
  • Hansbro PM; Department of Biotechnology, School of Engineering & Technology (SET), Sharda University, Greater Noida, Uttar Pradesh, 201310, India.
  • Chellappan DK; Department of Life Sciences, School of Basic Sciences and Research (SBSR), Sharda University, Knowledge Park III, Greater Noida-201310, Uttar Pradesh, India.
  • Dua K; School of Pharmaceutical Sciences, Lovely Professional University, Phagwara, Punjab, 144411, India.
Environ Sci Pollut Res Int ; 29(31): 46830-46847, 2022 Jul.
Article en En | MEDLINE | ID: mdl-35171422
Non-small cell lung cancer (NSCLC) is reported to have a high incidence rate and is one of the most prevalent types of cancer contributing towards 85% of all incidences of lung cancer. Berberine is an isoquinoline alkaloid which offers a broad range of therapeutical and pharmacological actions against cancer. However, extremely low water solubility and poor oral bioavailability have largely restricted its therapeutic applications. To overcome these limitations, we formulated berberine-loaded liquid crystalline nanoparticles (LCNs) and investigated their in vitro antiproliferative and antimigratory activity in human lung epithelial cancer cell line (A549). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), trypan blue staining, and colony forming assays were used to evaluate the anti-proliferative activity, while scratch wound healing assay and a modified Boyden chamber assay were carried out to determine the anti-migratory activity. We also investigated major proteins associated with lung cancer progression. The developed nanoparticles were found to have an average particle size of 181.3 nm with spherical shape, high entrapment efficiency (75.35%) and have shown sustained release behaviour. The most remarkable findings reported with berberine-loaded LCNs were significant suppression of proliferation, inhibition of colony formation, inhibition of invasion or migration via epithelial mesenchymal transition, and proliferation related proteins associated with cancer progression. Our findings suggest that anti-cancer compounds with the problem of poor solubility and bioavailability can be overcome by formulating them into nanotechnology-based delivery systems for better efficacy. Further in-depth investigations into anti-cancer mechanistic research will expand and strengthen the current findings of berberine-LCNs as a potential NSCLC treatment option.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Berberina / Carcinoma de Pulmón de Células no Pequeñas / Nanopartículas / Neoplasias Pulmonares Límite: Humans Idioma: En Revista: Environ Sci Pollut Res Int Asunto de la revista: SAUDE AMBIENTAL / TOXICOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Alemania
Texto completo
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Berberina / Carcinoma de Pulmón de Células no Pequeñas / Nanopartículas / Neoplasias Pulmonares Límite: Humans Idioma: En Revista: Environ Sci Pollut Res Int Asunto de la revista: SAUDE AMBIENTAL / TOXICOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Alemania