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The impacts of faecal subsampling on microbial compositional profiling.
Cox, Amanda J; Hughes, Lily; Nelson, Tiffanie M; Hatton-Jones, Kyle M; Ramsey, Rebecca; Cripps, Allan W; West, Nicholas P.
Afiliación
  • Cox AJ; School of Medical Science, Griffith Health Centre (G40), Griffith University, Parklands Drive, Southport, QLD, 4215, Australia. a.cox@griffith.edu.au.
  • Hughes L; Menzies Health Institute Queensland, Griffith University, Southport, QLD, Australia. a.cox@griffith.edu.au.
  • Nelson TM; School of Medical Science, Griffith Health Centre (G40), Griffith University, Parklands Drive, Southport, QLD, 4215, Australia.
  • Hatton-Jones KM; Menzies Health Institute Queensland, Griffith University, Southport, QLD, Australia.
  • Ramsey R; Queensland Cyber Infrastructure Foundation Ltd Bioinformatics, St Lucia, QLD, Australia.
  • Cripps AW; School of Medical Science, Griffith Health Centre (G40), Griffith University, Parklands Drive, Southport, QLD, 4215, Australia.
  • West NP; School of Medical Science, Griffith Health Centre (G40), Griffith University, Parklands Drive, Southport, QLD, 4215, Australia.
BMC Res Notes ; 15(1): 49, 2022 Feb 14.
Article en En | MEDLINE | ID: mdl-35164843
OBJECTIVE: Despite the move to at-home, small-volume collection kits to facilitate large population-based studies of faecal microbial compositional profiling, there remains limited reporting on potential impacts of faecal subsampling approaches on compositional profiles. This study aimed to compare the microbial composition from faecal subsamples (< 5 g) collected from the beginning and end of a single bowel movement in ten otherwise healthy adults (6 female, 4 male; age: 24-55 years). Microbial composition was determined by V3-V4 16s rRNA sequencing and compared between subsamples. RESULTS: There were no significant differences in OTU count (p = 0.32) or Shannon diversity index (p = 0.29) between the subsamples. Comparison of relative abundance for identified taxa revealed very few differences between subsamples. At the lower levels of taxonomic classification differences in abundance of the Bacillales (p = 0.02) and the Eubacteriaceae family (p = 0.03), and the Eubacterium genera (p = 0.03) were noted. The observation of consistent microbial compositional profiles between faecal subsamples from the beginning and end of a single bowel movement is an important outcome for study designs employing this approach to faecal sample collection. These findings provide assurance that use of a faecal subsample for microbial composition profiling is generally representative of the gut luminal contents more broadly.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Microbioma Gastrointestinal Tipo de estudio: Prognostic_studies Límite: Adult / Female / Humans / Male / Middle aged Idioma: En Revista: BMC Res Notes Año: 2022 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Microbioma Gastrointestinal Tipo de estudio: Prognostic_studies Límite: Adult / Female / Humans / Male / Middle aged Idioma: En Revista: BMC Res Notes Año: 2022 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Reino Unido