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Exposure to b-LED Light While Exerting Antimicrobial Activity on Gram-Negative and -Positive Bacteria Promotes Transient EMT-like Changes and Growth Arrest in Keratinocytes.
Terri, Michela; Mancianti, Nicoletta; Trionfetti, Flavia; Casciaro, Bruno; de Turris, Valeria; Raponi, Giammarco; Bontempi, Giulio; Montaldo, Claudia; Domenici, Alessandro; Menè, Paolo; Mangoni, Maria Luisa; Strippoli, Raffaele.
Afiliación
  • Terri M; Department of Molecular Medicine, Sapienza University of Rome, 00161 Rome, Italy.
  • Mancianti N; Gene Expression Laboratory, National Institute for Infectious Diseases, Lazzaro Spallanzani IRCCS, 00161 Rome, Italy.
  • Trionfetti F; Renal Unit, Department of Clinical and Molecular Medicine, Sant'Andrea University Hospital, Sapienza University of Rome, 00189 Rome, Italy.
  • Casciaro B; Department of Molecular Medicine, Sapienza University of Rome, 00161 Rome, Italy.
  • de Turris V; Gene Expression Laboratory, National Institute for Infectious Diseases, Lazzaro Spallanzani IRCCS, 00161 Rome, Italy.
  • Raponi G; Center for Life Nano- & Neuro-Science, Istituto Italiano di Tecnologia (IIT), 00161 Rome, Italy.
  • Bontempi G; Center for Life Nano- & Neuro-Science, Istituto Italiano di Tecnologia (IIT), 00161 Rome, Italy.
  • Montaldo C; Department of Public Health and Infectious Diseases, Sapienza University of Rome, 00185 Rome, Italy.
  • Domenici A; Department of Molecular Medicine, Sapienza University of Rome, 00161 Rome, Italy.
  • Menè P; Gene Expression Laboratory, National Institute for Infectious Diseases, Lazzaro Spallanzani IRCCS, 00161 Rome, Italy.
  • Mangoni ML; Gene Expression Laboratory, National Institute for Infectious Diseases, Lazzaro Spallanzani IRCCS, 00161 Rome, Italy.
  • Strippoli R; Renal Unit, Department of Clinical and Molecular Medicine, Sant'Andrea University Hospital, Sapienza University of Rome, 00189 Rome, Italy.
Int J Mol Sci ; 23(3)2022 Feb 08.
Article en En | MEDLINE | ID: mdl-35163819
While blue LED (b-LED) light is increasingly being studied for its cytotoxic activity towards bacteria in therapy of skin-related infections, its effects on eukaryotic cells plasticity are less well characterized. Moreover, since different protocols are often used, comparing the effect of b-LED towards both microorganisms and epithelial surfaces may be difficult. The aim of this study was to analyze, in the same experimental setting, both the bactericidal activity and the effects on human keratinocytes. Exposure to b-LED induced an intense cytocidal activity against Gram-positive (i.e, Staphylococcus aureus) and Gram-negative (i.e., Pseudomonas aeruginosa) bacteria associated with catheter-related infections. Treatment with b-LED of a human keratinocyte cell line induced a transient cell cycle arrest. At the molecular level, exposure to b-LED induced a transient downregulation of Cyclin D1 and an upregulation of p21, but not signs of apoptosis. Interestingly, a transient induction of phosphor-histone γ-H2Ax, which is associated with genotoxic damages, was observed. At the same time, keratinocytes underwent a transient epithelial to mesenchymal transition (EMT)-like phenotype, characterized by E-cadherin downregulation and SNAIL/SLUG induction. As a functional readout of EMT induction, a scratch assay was performed. Surprisingly, b-LED treatment provoked a delay in the scratch closure. In conclusion, we demonstrated that b-LED microbicidal activity is associated with complex responses in keratinocytes that certainly deserve further analysis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pseudomonas aeruginosa / Staphylococcus aureus / Queratinocitos / Puntos de Control del Ciclo Celular / Luz Tipo de estudio: Guideline Límite: Humans Idioma: En Revista: Int J Mol Sci Año: 2022 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pseudomonas aeruginosa / Staphylococcus aureus / Queratinocitos / Puntos de Control del Ciclo Celular / Luz Tipo de estudio: Guideline Límite: Humans Idioma: En Revista: Int J Mol Sci Año: 2022 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Suiza