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Induction of the Unfolded Protein Response at High Temperature in Saccharomyces cerevisiae.
Hata, Tatsuya; Ishiwata-Kimata, Yuki; Kimata, Yukio.
Afiliación
  • Hata T; Division of Biological Science, Graduate School of Science and Technology, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan.
  • Ishiwata-Kimata Y; Division of Biological Science, Graduate School of Science and Technology, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan.
  • Kimata Y; Division of Biological Science, Graduate School of Science and Technology, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan.
Int J Mol Sci ; 23(3)2022 Jan 31.
Article en En | MEDLINE | ID: mdl-35163590
Ire1 is an endoplasmic reticulum (ER)-located endoribonuclease that is activated in response to ER stress. In yeast Saccharomyces cerevisiae cells, Ire1 promotes HAC1-mRNA splicing to remove the intron sequence from the HAC1u mRNA ("u" stands for "uninduced"). The resulting mRNA, which is named HAC1i mRNA ("i" stands for "induced"), is then translated into a transcription factor that is involved in the unfolded protein response (UPR). In this study, we designed an oligonucleotide primer that specifically hybridizes to the exon-joint site of the HAC1i cDNA. This primer allowed us to perform real-time reverse transcription-PCR to quantify HAC1i mRNA abundance with high sensitivity. Using this method, we detected a minor induction of HAC1-mRNA splicing in yeast cells cultured at their maximum growth temperature of 39 °C. Based on our analyses of IRE1-gene mutant strains, we propose that when yeast cells are cultured at or near their maximum growth temperature, protein folding in the ER is disturbed, leading to a minor UPR induction that supports cellular growth.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Represoras / Saccharomyces cerevisiae / Empalme del ARN / Proteínas de Saccharomyces cerevisiae / Factores de Transcripción con Cremalleras de Leucina de Carácter Básico / Respuesta de Proteína Desplegada / Calor Idioma: En Revista: Int J Mol Sci Año: 2022 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Represoras / Saccharomyces cerevisiae / Empalme del ARN / Proteínas de Saccharomyces cerevisiae / Factores de Transcripción con Cremalleras de Leucina de Carácter Básico / Respuesta de Proteína Desplegada / Calor Idioma: En Revista: Int J Mol Sci Año: 2022 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Suiza