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Clickable report tags for identification of modified peptides by mass spectrometry.
Makarov, Dmytro; Telek, András; Becker, Tobias; von Wrisberg, Marie-Kristin; Schneider, Sabine; Kielkowski, Pavel.
Afiliación
  • Makarov D; Department of Chemistry, LMU Munich, Munich, Germany.
  • Telek A; Department of Chemistry, LMU Munich, Munich, Germany.
  • Becker T; Department of Applied Biotechnology, Budapest University of Technology and Economics, Budapest, Hungary.
  • von Wrisberg MK; Department of Chemistry, LMU Munich, Munich, Germany.
  • Schneider S; Department of Chemistry, LMU Munich, Munich, Germany.
  • Kielkowski P; Department of Chemistry, LMU Munich, Munich, Germany.
J Mass Spectrom ; 57(3): e4812, 2022 Jan 18.
Article en En | MEDLINE | ID: mdl-35156258
The identification and quantification of modified peptides are critical for the functional characterization of post-translational protein modifications (PTMs) to elucidate their biological function. Nowadays, quantitative mass spectrometry coupled with various bioinformatic pipelines has been successfully used for the determination of a wide range of PTMs. However, direct characterization of low abundant protein PTMs in bottom-up proteomic workflow remains challenging. Here, we present the synthesis and evaluation of tandem mass spectrometry tags (TMT) which are introduced via click-chemistry into peptides bearing alkyne handles. The fragmentation properties of the two mass tags were validated and used for screening in a model system and analysis of AMPylated proteins. The presented tags provide a valuable tool for diagnostic peak generation to increase confidence in the identification of modified peptides and potentially for direct peptide-PTM quantification from various experimental conditions.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies Idioma: En Revista: J Mass Spectrom Asunto de la revista: BIOQUIMICA Año: 2022 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies Idioma: En Revista: J Mass Spectrom Asunto de la revista: BIOQUIMICA Año: 2022 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Reino Unido