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Co-flow injection for serial crystallography at X-ray free-electron lasers.
Doppler, Diandra; Rabbani, Mohammad T; Letrun, Romain; Cruz Villarreal, Jorvani; Kim, Dai Hyun; Gandhi, Sahir; Egatz-Gomez, Ana; Sonker, Mukul; Chen, Joe; Koua, Faisal H M; Yang, Jayhow; Youssef, Mohamed; Mazalova, Victoria; Bajt, Sasa; Shelby, Megan L; Coleman, Matt A; Wiedorn, Max O; Knoska, Juraj; Schön, Silvan; Sato, Tokushi; Hunter, Mark S; Hosseinizadeh, Ahmad; Kuptiz, Christopher; Nazari, Reza; Alvarez, Roberto C; Karpos, Konstantinos; Zaare, Sahba; Dobson, Zachary; Discianno, Erin; Zhang, Shangji; Zook, James D; Bielecki, Johan; de Wijn, Raphael; Round, Adam R; Vagovic, Patrik; Kloos, Marco; Vakili, Mohammad; Ketawala, Gihan K; Stander, Natasha E; Olson, Tien L; Morin, Katherine; Mondal, Jyotirmory; Nguyen, Jonathan; Meza-Aguilar, José Domingo; Kodis, Gerdenis; Vaiana, Sara; Martin-Garcia, Jose M; Mariani, Valerio; Schwander, Peter; Schmidt, Marius.
Afiliación
  • Doppler D; School of Molecular Sciences, Arizona State University, Tempe, Arizona, USA.
  • Rabbani MT; Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, Arizona, USA.
  • Letrun R; School of Molecular Sciences, Arizona State University, Tempe, Arizona, USA.
  • Cruz Villarreal J; Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, Arizona, USA.
  • Kim DH; European XFEL, Schenefeld, Germany.
  • Gandhi S; School of Molecular Sciences, Arizona State University, Tempe, Arizona, USA.
  • Egatz-Gomez A; Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, Arizona, USA.
  • Sonker M; School of Molecular Sciences, Arizona State University, Tempe, Arizona, USA.
  • Chen J; Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, Arizona, USA.
  • Koua FHM; School of Molecular Sciences, Arizona State University, Tempe, Arizona, USA.
  • Yang J; Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, Arizona, USA.
  • Youssef M; School of Molecular Sciences, Arizona State University, Tempe, Arizona, USA.
  • Mazalova V; Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, Arizona, USA.
  • Bajt S; School of Molecular Sciences, Arizona State University, Tempe, Arizona, USA.
  • Shelby ML; Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, Arizona, USA.
  • Coleman MA; Department of Physics, Arizona State University, Tempe, Arizona, USA.
  • Wiedorn MO; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron (DESY), Hamburg, Germany.
  • Knoska J; Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, Arizona, USA.
  • Schön S; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron (DESY), Hamburg, Germany.
  • Sato T; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron (DESY), Hamburg, Germany.
  • Hunter MS; Hamburg Center for Ultrafast Imaging, Hamburg, Germany.
  • Hosseinizadeh A; Deutsches Elektronen-Synchrotron (DESY), Hamburg, Germany.
  • Kuptiz C; Lawrence Livermore National Laboratory (LLNL), Livermore, California, USA.
  • Nazari R; Lawrence Livermore National Laboratory (LLNL), Livermore, California, USA.
  • Alvarez RC; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron (DESY), Hamburg, Germany.
  • Karpos K; Hamburg Center for Ultrafast Imaging, Hamburg, Germany.
  • Zaare S; Department of Physics, Universität Hamburg, Hamburg, Germany.
  • Dobson Z; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron (DESY), Hamburg, Germany.
  • Discianno E; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron (DESY), Hamburg, Germany.
  • Zhang S; European XFEL, Schenefeld, Germany.
  • Zook JD; Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
  • Bielecki J; Department of Physics, University of Wisconsin-Milwaukee, Milwaukee, Wisconsin, USA.
  • de Wijn R; Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
  • Round AR; Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, Arizona, USA.
  • Vagovic P; Department of Physics, Arizona State University, Tempe, Arizona, USA.
  • Kloos M; Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, Arizona, USA.
  • Vakili M; Department of Physics, Arizona State University, Tempe, Arizona, USA.
  • Ketawala GK; Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, Arizona, USA.
  • Stander NE; Department of Physics, Arizona State University, Tempe, Arizona, USA.
  • Olson TL; Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, Arizona, USA.
  • Morin K; Department of Physics, Arizona State University, Tempe, Arizona, USA.
  • Mondal J; School of Molecular Sciences, Arizona State University, Tempe, Arizona, USA.
  • Nguyen J; Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, Arizona, USA.
  • Meza-Aguilar JD; Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, Arizona, USA.
  • Kodis G; School of Molecular Sciences, Arizona State University, Tempe, Arizona, USA.
  • Vaiana S; Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, Arizona, USA.
  • Martin-Garcia JM; School of Molecular Sciences, Arizona State University, Tempe, Arizona, USA.
  • Mariani V; Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, Arizona, USA.
  • Schwander P; European XFEL, Schenefeld, Germany.
  • Schmidt M; European XFEL, Schenefeld, Germany.
J Appl Crystallogr ; 55(Pt 1): 1-13, 2022 Feb 01.
Article en En | MEDLINE | ID: mdl-35153640
Serial femtosecond crystallography (SFX) is a powerful technique that exploits X-ray free-electron lasers to determine the structure of macro-molecules at room temperature. Despite the impressive exposition of structural details with this novel crystallographic approach, the methods currently available to introduce crystals into the path of the X-ray beam sometimes exhibit serious drawbacks. Samples requiring liquid injection of crystal slurries consume large quantities of crystals (at times up to a gram of protein per data set), may not be compatible with vacuum configurations on beamlines or provide a high background due to additional sheathing liquids present during the injection. Proposed and characterized here is the use of an immiscible inert oil phase to supplement the flow of sample in a hybrid microfluidic 3D-printed co-flow device. Co-flow generation is reported with sample and oil phases flowing in parallel, resulting in stable injection conditions for two different resin materials experimentally. A numerical model is presented that adequately predicts these flow-rate conditions. The co-flow generating devices reduce crystal clogging effects, have the potential to conserve protein crystal samples up to 95% and will allow degradation-free light-induced time-resolved SFX.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: J Appl Crystallogr Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: J Appl Crystallogr Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos