Local administration of low doses of exogenous BMP2 and leptin promotes ectopic bone regeneration in leptin-deficient mice.
Biomed Mater Eng
; 33(4): 303-313, 2022.
Article
en En
| MEDLINE
| ID: mdl-35147528
BACKGROUND: Obesity and leptin deficiency are associated with compromised bone regeneration. OBJECTIVE: This study aims to investigate the role of locally administrated low-dose BMP2+leptin on bone regeneration in leptin-deficient obese (ob/ob) mice. METHODS: Wildtype (WT) and ob/ob mice were divided into 3 groups (4 mice/group): BMP2 (5 µg) group, BMP2+low-dose leptin (1 µg) group, and BMP2+high-dose leptin (2.5 µg) group. WT mice were used as control mice. An equal size absorbable collagen sponge was prepared by loading the BMP2 or/and leptin and implanted subcutaneously. After 19 days, samples were collected and analyzed by micro-CT and H&E staining. RESULTS: No significant difference in bone regeneration among the three groups in WT mice. Quantification of newly formed bone parameters from micro-CT and H&E staining showed that low-dose BMP2 treatment formed less new bone in ob/ob mice compared to WT. BMP2+low-dose leptin treatment substantially rescued the compromised bone regeneration in ob/ob mice up to the level in WT mice. However, the BMP2 and high dose of leptin failed to rescue the compromised bone regeneration in ob/ob mice. CONCLUSION: Our findings suggest that a combination of the low-dose BMP2 and leptin could be a strategy to promote osteogenesis in obese populations with leptin deficiency.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Regeneración Ósea
/
Leptina
/
Proteína Morfogenética Ósea 2
/
Obesidad
Límite:
Animals
Idioma:
En
Revista:
Biomed Mater Eng
Asunto de la revista:
BIOTECNOLOGIA
/
ENGENHARIA BIOMEDICA
Año:
2022
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
Países Bajos