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Genome-edited powdery mildew resistance in wheat without growth penalties.
Li, Shengnan; Lin, Dexing; Zhang, Yunwei; Deng, Min; Chen, Yongxing; Lv, Bin; Li, Boshu; Lei, Yuan; Wang, Yanpeng; Zhao, Long; Liang, Yueting; Liu, Jinxing; Chen, Kunling; Liu, Zhiyong; Xiao, Jun; Qiu, Jin-Long; Gao, Caixia.
Afiliación
  • Li S; State Key Laboratory of Plant Genomics, Institute of Microbiology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, China.
  • Lin D; State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, China.
  • Zhang Y; Center for Genome Editing, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
  • Deng M; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China.
  • Chen Y; State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, China.
  • Lv B; Center for Genome Editing, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
  • Li B; State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, China.
  • Lei Y; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China.
  • Wang Y; State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, China.
  • Zhao L; State Key Laboratory of Plant Genomics, Institute of Microbiology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, China.
  • Liang Y; CAS Center for Excellence in Biotic Interactions, University of Chinese Academy of Sciences, Beijing, China.
  • Liu J; State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, China.
  • Chen K; Center for Genome Editing, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
  • Liu Z; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China.
  • Xiao J; State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, China.
  • Qiu JL; Center for Genome Editing, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
  • Gao C; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China.
Nature ; 602(7897): 455-460, 2022 02.
Article en En | MEDLINE | ID: mdl-35140403
Disruption of susceptibility (S) genes in crops is an attractive breeding strategy for conferring disease resistance1,2. However, S genes are implicated in many essential biological functions and deletion of these genes typically results in undesired pleiotropic effects1. Loss-of-function mutations in one such S gene, Mildew resistance locus O (MLO), confers durable and broad-spectrum resistance to powdery mildew in various plant species2,3. However, mlo-associated resistance is also accompanied by growth penalties and yield losses3,4, thereby limiting its widespread use in agriculture. Here we describe Tamlo-R32, a mutant with a 304-kilobase pair targeted deletion in the MLO-B1 locus of wheat that retains crop growth and yields while conferring robust powdery mildew resistance. We show that this deletion results in an altered local chromatin landscape, leading to the ectopic activation of Tonoplast monosaccharide transporter 3 (TaTMT3B), and that this activation alleviates growth and yield penalties associated with MLO disruption. Notably, the function of TMT3 is conserved in other plant species such as Arabidopsis thaliana. Moreover, precision genome editing facilitates the rapid introduction of this mlo resistance allele (Tamlo-R32) into elite wheat varieties. This work demonstrates the ability to stack genetic changes to rescue growth defects caused by recessive alleles, which is critical for developing high-yielding crop varieties with robust and durable disease resistance.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ascomicetos / Triticum / Genoma de Planta / Resistencia a la Enfermedad / Edición Génica Idioma: En Revista: Nature Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ascomicetos / Triticum / Genoma de Planta / Resistencia a la Enfermedad / Edición Génica Idioma: En Revista: Nature Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido