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Cell biological analysis reveals an essential role for Pfcerli2 in erythrocyte invasion by malaria parasites.
Liffner, Benjamin; Balbin, Juan Miguel; Shami, Gerald J; Siddiqui, Ghizal; Strauss, Jan; Frölich, Sonja; Heinemann, Gary K; Edwards, Ella May; Alder, Arne; Wichers, Jan Stephan; Creek, Darren J; Tilley, Leann; Dixon, Matthew W A; Gilberger, Tim-Wolf; Wilson, Danny W.
Afiliación
  • Liffner B; Research Centre for Infectious Diseases, School of Biological Sciences, University of Adelaide, Adelaide, 5005, Australia.
  • Balbin JM; Research Centre for Infectious Diseases, School of Biological Sciences, University of Adelaide, Adelaide, 5005, Australia.
  • Shami GJ; Department of Biochemistry and Pharmacology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Melbourne, VIC, 3010, Australia.
  • Siddiqui G; Dept Drug Delivery, Disposition and Dynamics, Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, VIC, 3052, Australia.
  • Strauss J; Centre for Structural Systems Biology, 22607, Hamburg, Germany.
  • Frölich S; Bernhard Nocht Institute for Tropical Medicine, 20359, Hamburg, Germany.
  • Heinemann GK; Biology Department, University of Hamburg, 20146, Hamburg, Germany.
  • Edwards EM; GEOMAR Helmholtz Centre for Ocean Research Kiel, 24105, Kiel, Germany.
  • Alder A; Research Centre for Infectious Diseases, School of Biological Sciences, University of Adelaide, Adelaide, 5005, Australia.
  • Wichers JS; Experimental Therapeutics Laboratory, Clinical and Health Science Unit, University of South Australia, Adelaide, SA, 5000, Australia.
  • Creek DJ; Research Centre for Infectious Diseases, School of Biological Sciences, University of Adelaide, Adelaide, 5005, Australia.
  • Tilley L; Centre for Structural Systems Biology, 22607, Hamburg, Germany.
  • Dixon MWA; Bernhard Nocht Institute for Tropical Medicine, 20359, Hamburg, Germany.
  • Gilberger TW; Biology Department, University of Hamburg, 20146, Hamburg, Germany.
  • Wilson DW; Centre for Structural Systems Biology, 22607, Hamburg, Germany.
Commun Biol ; 5(1): 121, 2022 02 09.
Article en En | MEDLINE | ID: mdl-35140336
Merozoite invasion of host red blood cells (RBCs) is essential for survival of the human malaria parasite Plasmodium falciparum. Proteins involved with RBC binding and invasion are secreted from dual-club shaped organelles at the apical tip of the merozoite called the rhoptries. Here we characterise P. falciparum Cytosolically Exposed Rhoptry Leaflet Interacting protein 2 (PfCERLI2), as a rhoptry bulb protein that is essential for merozoite invasion. Phylogenetic analyses show that cerli2 arose through an ancestral gene duplication of cerli1. We show that PfCERLI2 is essential for blood-stage growth and localises to the cytosolic face of the rhoptry bulb. Inducible knockdown of PfCERLI2 led to a proportion of merozoites failing to invade and was associated with elongation of the rhoptry organelle during merozoite development and inhibition of rhoptry antigen processing. These findings identify PfCERLI2 as a protein that has key roles in rhoptry biology during merozoite invasion.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Parásitos / Malaria Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Commun Biol Año: 2022 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Parásitos / Malaria Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Commun Biol Año: 2022 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Reino Unido