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Non-diarrhoeic pigs as source of highly virulent and multidrug-resistant non-typhoidal Salmonella.
Karabasanavar, Nagappa; Sivaraman, G K; S P, Satheesha; Nair, Archana S; Vijayan, Ardhra; Rajan, Vineeth; P S, Girish.
Afiliación
  • Karabasanavar N; Department of Veterinary Public Health & Epidemiology, Veterinary College (Karnataka Veterinary, Animal & Fisheries Sciences University), Vidyanagar, Hassan, 573 202, Karnataka, India. pub.nag@gmail.com.
  • Sivaraman GK; Microbiology, Fermentation & Biotechnology Division, ICAR-Central Institute of Fisheries Technology, Cochin, 682 029, Kerala, India.
  • S P S; Department of Veterinary Public Health & Epidemiology, Veterinary College (Karnataka Veterinary, Animal & Fisheries Sciences University), Vidyanagar, Hassan, 573 202, Karnataka, India.
  • Nair AS; Department of Veterinary Public Health & Epidemiology, Veterinary College (Karnataka Veterinary, Animal & Fisheries Sciences University), Vidyanagar, Hassan, 573 202, Karnataka, India.
  • Vijayan A; Microbiology, Fermentation & Biotechnology Division, ICAR-Central Institute of Fisheries Technology, Cochin, 682 029, Kerala, India.
  • Rajan V; Microbiology, Fermentation & Biotechnology Division, ICAR-Central Institute of Fisheries Technology, Cochin, 682 029, Kerala, India.
  • P S G; ICAR National Research Centre On Meat, Hyderabad, 500 092, Telangana, India.
Braz J Microbiol ; 53(2): 1039-1049, 2022 Jun.
Article en En | MEDLINE | ID: mdl-35128626
Food-producing animals act as reservoirs of non-typhoidal Salmonella (NTS) serovars with potential food safety and public health implications. The present cross-sectional study aimed at determining the prevalence of Salmonella serotypes in non-diarrhoeic pigs and characterizing the isolates using molecular tools. Salmonella isolates (n = 22) recovered from faecal samples of 194 randomly selected pigs were characterized for virulence and antimicrobial resistance and subtyped using XbaI-PFGE. The prevalence of Salmonella in apparently healthy non-diarrhoeic pigs was 11.3% (95%CI, 4.3-19.5%), with S. Weltevreden (81.8%) and S. Enteritidis (18.2%) being the serotypes detected. Salmonella isolates harboured virulence genes such as invA (100%), stn (100%), spvR/spvC (86.3%) and fimA (22.7%). Phenotypically, isolates showed sensitivity to chloramphenicol, levofloxacin and ciprofloxacin and resistance to tetracycline and ampicillin (100%), streptomycin (86.4%), amoxicillin-clavulanate (63.6%), cefotaxime (22.7%) and ceftriaxone (9.1%). Notably, 18.2% isolates were multidrug-resistant (≥ 3 antimicrobial class) with multiple antimicrobial resistance (MAR) index of 0.56-0.67 (18.2%), 0.44 (45.5%), 0.33 (31.8%) and 0.22 (4.5%). Genotypically, isolates carried various antibiotic resistance genes: ESBL (blaTEM and blaOXA), aminoglycoside (strA, strB and aadA1), sulphonamide (sul1, sul2 and dfrA1), tetracycline (tetA and tetB) and plasmid AmpC beta-lactamase (ACC, FOX, MOX, DHA, CIT and EBC). The present investigation emphasizes the epidemiological significance of PFGE typing in the detection of emerging strains of highly virulent and multidrug-resistant S. Weltevreden and S. Enteritidis in non-diarrhoeic pigs that pose serious public health implications in the pork supply chain environment. More extensive longitudinal study is warranted to provide epidemiological links between environmental reservoirs and animal and human infections in piggery settings.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Salmonelosis Animal / Farmacorresistencia Bacteriana Múltiple Tipo de estudio: Observational_studies / Prevalence_studies / Risk_factors_studies Límite: Animals Idioma: En Revista: Braz J Microbiol Año: 2022 Tipo del documento: Article País de afiliación: India Pais de publicación: Brasil
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Salmonelosis Animal / Farmacorresistencia Bacteriana Múltiple Tipo de estudio: Observational_studies / Prevalence_studies / Risk_factors_studies Límite: Animals Idioma: En Revista: Braz J Microbiol Año: 2022 Tipo del documento: Article País de afiliación: India Pais de publicación: Brasil