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Acute but Not Chronic Central Administration of the Neuropeptide 26RFa (QRFP) Improves Glucose Homeostasis in Obese/Diabetic Mice.
Le Solliec, Marie-Anne; Arabo, Arnaud; Takhlidjt, Saloua; Maucotel, Julie; Devère, Mélodie; Riancho, Julien; Berrahmoune, Hind; do Rego, Jean-Luc; do Rego, Jean-Claude; Bénani, Alexandre; Nedelec, Emmanuelle; Lefranc, Benjamin; Leprince, Jérôme; Anouar, Youssef; Picot, Marie; Chartrel, Nicolas; Prévost, Gaëtan.
Afiliación
  • Le Solliec MA; Institute for Research and Innovation in Biomedicine (IRIB), UNIROUEN, INSERM U1239, Laboratory of Neuronal and Neuroendocrine Differentiation and Communication (DC2N), Normandie University, Rouen, France.
  • Arabo A; Institute for Research and Innovation in Biomedicine (IRIB), Department of Biological Resources (SRB), UNIROUEN, Normandie University, Rouen, France.
  • Takhlidjt S; Institute for Research and Innovation in Biomedicine (IRIB), UNIROUEN, INSERM U1239, Laboratory of Neuronal and Neuroendocrine Differentiation and Communication (DC2N), Normandie University, Rouen, France.
  • Maucotel J; Institute for Research and Innovation in Biomedicine (IRIB), Department of Biological Resources (SRB), UNIROUEN, Normandie University, Rouen, France.
  • Devère M; Institute for Research and Innovation in Biomedicine (IRIB), UNIROUEN, INSERM U1239, Laboratory of Neuronal and Neuroendocrine Differentiation and Communication (DC2N), Normandie University, Rouen, France.
  • Riancho J; Institute for Research and Innovation in Biomedicine (IRIB), UNIROUEN, INSERM U1239, Laboratory of Neuronal and Neuroendocrine Differentiation and Communication (DC2N), Normandie University, Rouen, France.
  • Berrahmoune H; Institute for Research and Innovation in Biomedicine (IRIB), UNIROUEN, INSERM U1239, Laboratory of Neuronal and Neuroendocrine Differentiation and Communication (DC2N), Normandie University, Rouen, France.
  • do Rego JL; Institute for Research and Innovation in Biomedecine (IRIB), UNIROUEN, Animal Behaviour Platform SCAC, Normandie University, Rouen, France.
  • do Rego JC; Institute for Research and Innovation in Biomedecine (IRIB), UNIROUEN, Animal Behaviour Platform SCAC, Normandie University, Rouen, France.
  • Bénani A; Center for Taste and Feeding Behaviour, CNRS (UMR6265), INRA (UMR1324), AgroSup Dijon, Université de Bourgogne-Franche Comté, Dijon, France.
  • Nedelec E; Center for Taste and Feeding Behaviour, CNRS (UMR6265), INRA (UMR1324), AgroSup Dijon, Université de Bourgogne-Franche Comté, Dijon, France.
  • Lefranc B; Institute for Research and Innovation in Biomedicine (IRIB), UNIROUEN, INSERM U1239, Laboratory of Neuronal and Neuroendocrine Differentiation and Communication (DC2N), Normandie University, Rouen, France.
  • Leprince J; Cell Imaging Platform of Normandy, Normandie University, Rouen, France.
  • Anouar Y; Institute for Research and Innovation in Biomedicine (IRIB), UNIROUEN, INSERM U1239, Laboratory of Neuronal and Neuroendocrine Differentiation and Communication (DC2N), Normandie University, Rouen, France.
  • Picot M; Cell Imaging Platform of Normandy, Normandie University, Rouen, France.
  • Chartrel N; Institute for Research and Innovation in Biomedicine (IRIB), UNIROUEN, INSERM U1239, Laboratory of Neuronal and Neuroendocrine Differentiation and Communication (DC2N), Normandie University, Rouen, France.
  • Prévost G; Institute for Research and Innovation in Biomedicine (IRIB), UNIROUEN, INSERM U1239, Laboratory of Neuronal and Neuroendocrine Differentiation and Communication (DC2N), Normandie University, Rouen, France.
Neuroendocrinology ; 112(11): 1104-1115, 2022.
Article en En | MEDLINE | ID: mdl-35093951
INTRODUCTION: The aim of the study is to investigate whether acute or chronic central administration of the hypothalamic neuropeptide 26RFa may ameliorate the glycemic control of obese/diabetic mice. METHODS: Mice were treated for 4 months with a high-fat (HF) diet and received a single i.c.v. injection of 26RFa (3 µg) or a chronic i.c.v. administration of the peptide during 28 days via osmotic minipumps (25 µg/day). i.p. and oral glucose (GLU) tolerance tests, insulin (INS) tolerance test, glucose-stimulated insulin secretion (GSIS), food/water intake, horizontal/vertical activity, energy expenditure, meal pattern, and whole-body composition were monitored. In addition, 26RFa and GPR103 mRNA expressions as well as plasma 26RFa levels were evaluated by RT-QPCR and radioimmunoassay. RESULTS: Acute administration of 26RFa in HF mice induced a robust antihyperglycemic effect by enhancing INS secretion, whereas chronic administration of the neuropeptide is unable to improve glucose homeostasis in these obese/diabetogenic conditions. By contrast, chronic 26RFa treatment induced an increase of the body weight accompanied with an enhanced food intake and a decreased energy expenditure. Finally, we show that the HF diet does not alter the hypothalamic expression of the 26RFa/GPR103 neuropeptidergic system nor the levels of circulating 26RFa. CONCLUSION: Our data indicate that the central beneficial effect of 26RFa on glucose homeostasis, by potentiating GSIS, is preserved in HF mice. However, chronic administration of the neuropeptide is unable to balance glycemia in these pathophysiological conditions, suggesting that the hypothalamic 26RFa/GPR103 neuropeptidergic system mainly affects short-term regulation of glucose metabolism.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neuropéptidos / Diabetes Mellitus Experimental / Insulinas Límite: Animals Idioma: En Revista: Neuroendocrinology Año: 2022 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neuropéptidos / Diabetes Mellitus Experimental / Insulinas Límite: Animals Idioma: En Revista: Neuroendocrinology Año: 2022 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Suiza