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WDR62 regulates mouse oocyte meiotic maturation related to p-JNK and H3K9 trimethylation.
Wang, Yong-Sheng; Chen, Chao; Ahmad, Muhammad Jamil; Chen, Fan; Ding, Zhi-Ming; Yang, Sheng-Ji; Chen, Yang-Wu; Duan, Ze-Qun; Liu, Ming; Liang, Ai-Xin; He, Chang-Jiu; Hua, Guo-Hua; Huo, Li-Jun.
Afiliación
  • Wang YS; College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; Na
  • Chen C; College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; Na
  • Ahmad MJ; College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; Na
  • Chen F; College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; Na
  • Ding ZM; College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; Na
  • Yang SJ; College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; Na
  • Chen YW; College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; Na
  • Duan ZQ; College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; Na
  • Liu M; College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; Na
  • Liang AX; College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; Na
  • He CJ; College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; Na
  • Hua GH; College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; Na
  • Huo LJ; College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; Na
Int J Biochem Cell Biol ; 144: 106169, 2022 03.
Article en En | MEDLINE | ID: mdl-35093571
WDR62 (WD40-repeat protein 62) participates in diverse biological process, especially mitotic spindle organization via regulating centriole biogenesis and the function of centriole-associated protein. However, the role of WDR62 exerts in spindle assembly and meiotic progression control in oocytes lacking typical centrosomes remains obscure. In a previous study, we reported that WDR62 is involved in spindle migration and asymmetric cytokinesis in mouse oocyte meiosis. In the current study, another novel function of WDR62 regulating cell cycle progression through meiotic spindle formation during oocyte meiotic maturation was found. Knockdown of WDR62 through siRNA microinjection disrupted the meiotic cell cycle and induced metaphase-I (MI) arrest coupled with severe spindle abnormality, chromosome misalignment, and aneuploid generation. Moreover, WDR62 depletion induced defective kinetochore-microtubule attachments (K-MT) and activated spindle assembly checkpoint (SAC), which could trigger the arrest of meiotic progression. Further study demonstrated that depletion of WDR62 was associated with an aberrant location of p-JNK and reduced its expression level; concomitantly, status of H3K9 trimethylation was also altered. In addition, phenotypes similar to WDR62 depletion were observed during the function-loss analysis of p-JNK using a specific inhibitor (SP600125), which signifies that WDR62 is important for spindle organization and meiotic progression, and this function might be via its regulation of p-JNK. In conclusion, this study revealed that WDR62 functions in multiple ways during oocyte meiotic maturation, which could be related to p-JNK and H3K9 trimethylation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Meiosis / Huso Acromático Límite: Animals Idioma: En Revista: Int J Biochem Cell Biol Asunto de la revista: BIOQUIMICA Año: 2022 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Meiosis / Huso Acromático Límite: Animals Idioma: En Revista: Int J Biochem Cell Biol Asunto de la revista: BIOQUIMICA Año: 2022 Tipo del documento: Article Pais de publicación: Países Bajos