Your browser doesn't support javascript.
loading
Karyotype asymmetry in Cuscuta L. subgenus Pachystigma reflects its repeat DNA composition.
Ibiapino, Amalia; Báez, Mariana; García, Miguel A; Costea, Mihai; Stefanovic, Sasa; Pedrosa-Harand, Andrea.
Afiliación
  • Ibiapino A; Laboratory of Plant Cytogenetics and Evolution, Department of Botany, Federal University of Pernambuco, Recife, Brazil.
  • Báez M; Laboratory of Plant Cytogenetics and Evolution, Department of Botany, Federal University of Pernambuco, Recife, Brazil.
  • García MA; Plant Breeding Department, University of Bonn, Bonn, Germany.
  • Costea M; Real Jardín Botanico-CSIC, Madrid, Spain.
  • Stefanovic S; Department of Biology, Wilfrid Laurier University, Waterloo, Ontario, Canada.
  • Pedrosa-Harand A; Department of Biology, University of Toronto Mississauga, Mississauga, Ontario, Canada.
Chromosome Res ; 30(1): 91-107, 2022 03.
Article en En | MEDLINE | ID: mdl-35089455
Cuscuta is a cytogenetically diverse genus, with karyotypes varying 18-fold in chromosome number and 127-fold in genome size. Each of its four subgenera also presents particular chromosomal features, such as bimodal karyotypes in Pachystigma. We used low coverage sequencing of the Cuscuta nitida genome (subgenus Pachystigma), as well as chromosome banding and molecular cytogenetics of three subgenus representatives, to understand the origin of bimodal karyotypes. All three species, C. nitida, C. africana (2n = 28) and C. angulata (2n = 30), showed heterochromatic bands mainly in the largest chromosome pairs. Eighteen satellite DNAs were identified in C. nitida genome, two showing similarity to mobile elements. The most abundant were present at the largest pairs, as well as the highly abundant ribosomal DNAs. The most abundant Ty1/Copia and Ty3/Gypsy elements were also highly enriched in the largest pairs, except for the Ty3/Gypsy CRM, which also labelled the pericentromeric regions of the smallest chromosomes. This accumulation of repetitive DNA in the larger pairs indicates that these sequences are largely responsible for the formation of bimodal karyotypes in the subgenus Pachystigma. The repetitive DNA fraction is directly linked to karyotype evolution in Cuscuta.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cuscuta Idioma: En Revista: Chromosome Res Asunto de la revista: BIOLOGIA MOLECULAR Año: 2022 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cuscuta Idioma: En Revista: Chromosome Res Asunto de la revista: BIOLOGIA MOLECULAR Año: 2022 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Países Bajos