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Genotypic detection of metronidazole and clarithromycin resistance in dyspeptic patients with helicobacter pylori.
Nemr, Nader; Kishk, Rania M; Salem, Ayman; Abdalla, Mohamed O; Abd El-Fadeal, Noha M; Soliman, Nashaat M.
Afiliación
  • Nemr N; Endemic and Infectious Diseases Department, Faculty of Medicine, Suez Canal University, Ismailia, 4155, Egypt.
  • Kishk RM; Medical Microbiology and Immunology Department, Faculty of Medicine, Suez Canal University, Ismailia, 4155, Egypt. raniakishk@med.suez.edu.eg.
  • Salem A; Internal Medicine Department, Faculty of Medicine, Suez Canal University, Ismailia, 4155, Egypt.
  • Abdalla MO; Clinical Pathology Department, Faculty of Medicine, Suez Canal University, Ismailia, 4155, Egypt.
  • Abd El-Fadeal NM; Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Suez Canal University, Ismailia, 4155, Egypt.
  • Soliman NM; Endemic and Infectious Diseases Department, Faculty of Medicine, Suez Canal University, Ismailia, 4155, Egypt.
Environ Sci Pollut Res Int ; 29(27): 41467-41474, 2022 Jun.
Article en En | MEDLINE | ID: mdl-35088259
In Egypt, antibiotic sensitivity analysis for Helicobacter pylori is not routinely performed. We aimed to identify the clarithromycin and metronidazole resistance directly from gastric biopsies for better guide treatment regimens. This cross-sectional descriptive study included 75 adult dyspeptic patients referred to the upper endoscopy unit in Suez Canal University Hospital, Ismailia, Egypt. Gastric biopsies were taken for rapid urease test (RUT) and cultured on brucella agar with antibiotic supplements. Genomic DNA was extracted directly from the specimen, and PCR was performed for direct detection of H. pylori. Also, to explore clarithromycin and metronidazole resistance, mutations in the 23S rRNA gene and the rdxA gene were investigated. We found that 60 samples were positive to RUT (80%), and only 4 samples were positive by culture. UreC gene was detected in 45 specimens. Meanwhile, 26 isolates were contained mutations at positions 2142 and 2143. Amplification of the metronidazole rdx gene was performed by conventional PCR. Out of 45 isolates, DNA sequence analysis of PCR product showed the wild type (ACA) in 9 isolates, while the mutant type (ATA) was detected in 28 isolates. We found a significant proportion of clarithromycin and metronidazole resistance among H. pylori infected patients in our region.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Helicobacter pylori / Infecciones por Helicobacter Tipo de estudio: Diagnostic_studies / Observational_studies / Prevalence_studies / Risk_factors_studies Límite: Adult / Humans Idioma: En Revista: Environ Sci Pollut Res Int Asunto de la revista: SAUDE AMBIENTAL / TOXICOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: Egipto Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Helicobacter pylori / Infecciones por Helicobacter Tipo de estudio: Diagnostic_studies / Observational_studies / Prevalence_studies / Risk_factors_studies Límite: Adult / Humans Idioma: En Revista: Environ Sci Pollut Res Int Asunto de la revista: SAUDE AMBIENTAL / TOXICOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: Egipto Pais de publicación: Alemania