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Designing a Sequence-Based Method for Identifying 14 High-Risk Carcinogenic HPV Types in Multiple Infections.
Alimardani, Arezou; Dastmalchi, Narges; Rahimi, Hamzeh; Safaralizadeh, Reza.
Afiliación
  • Alimardani A; Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran.
  • Dastmalchi N; Department of Biology, University College of Nabi Akram, Tabriz, Iran.
  • Rahimi H; Pasteur Institute of Iran, Tehran, Iran.
  • Safaralizadeh R; Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran.
Infect Disord Drug Targets ; 22(3): e270122200585, 2022.
Article en En | MEDLINE | ID: mdl-35086459
BACKGROUND: HPV tests have significant drawbacks in terms of detecting and differentiating types of the virus. PCR techniques provide timely and necessary results for patient care with high quality, sensitivity, and reasonable cost. METHODS: The sensitivity of PCR depends on the primers. In this study, a method was designed that exploited PCR with designed primers (ScTd) by changing the annealing temperature (Ta) along with Sanger sequencing for pap smear samples. Sanger sequencing has confirmed that ScTd primers have a relative differentiation power using PCR. The primers caused a relative differentiation by PCR. In the pap smear sample 22 with contamination of types 16, 31, and 45, confirmed by dot blot hybridization, type 16 was not amplified at the specific Ta. Moreover, the band was observed at low Ta. RESULTS: Sanger sequencing showed that type 16 was detected instead of type 52. Sequencing the heterozygous bands in multiple infections also led to the identification of different types. Moreover, with a combination of 7 pairs of primers, HPV types can be detected in multiple infections by PCR. CONCLUSION: As compared with the clinical dot blot hybridization technique, the utilization of complementary PCR and sequencing methods with designed primers can provide a higher positive predictive value in the detection of high-risk types.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias del Cuello Uterino / Infecciones por Papillomavirus Tipo de estudio: Diagnostic_studies / Etiology_studies / Prognostic_studies / Risk_factors_studies Límite: Female / Humans Idioma: En Revista: Infect Disord Drug Targets Asunto de la revista: DOENCAS TRANSMISSIVEIS / TERAPIA POR MEDICAMENTOS Año: 2022 Tipo del documento: Article País de afiliación: Irán Pais de publicación: Emiratos Árabes Unidos
Texto completo
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias del Cuello Uterino / Infecciones por Papillomavirus Tipo de estudio: Diagnostic_studies / Etiology_studies / Prognostic_studies / Risk_factors_studies Límite: Female / Humans Idioma: En Revista: Infect Disord Drug Targets Asunto de la revista: DOENCAS TRANSMISSIVEIS / TERAPIA POR MEDICAMENTOS Año: 2022 Tipo del documento: Article País de afiliación: Irán Pais de publicación: Emiratos Árabes Unidos