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Adaptation of the periplasm to maintain spatial constraints essential for cell envelope processes and cell viability.
Mandela, Eric; Stubenrauch, Christopher J; Ryoo, David; Hwang, Hyea; Cohen, Eli J; Torres, Von L; Deo, Pankaj; Webb, Chaille T; Huang, Cheng; Schittenhelm, Ralf B; Beeby, Morgan; Gumbart, J C; Lithgow, Trevor; Hay, Iain D.
Afiliación
  • Mandela E; Infection & Immunity Program, Biomedicine Discovery Institute and Department of Microbiology, Monash University, Clayton, Australia.
  • Stubenrauch CJ; Infection & Immunity Program, Biomedicine Discovery Institute and Department of Microbiology, Monash University, Clayton, Australia.
  • Ryoo D; Interdisciplinary Bioengineering Graduate Program, Georgia Institute of Technology, Atlanta, United States.
  • Hwang H; School of Materials Science and Engineering, Georgia Institute of Technology, Atlanta, United States.
  • Cohen EJ; Department of Life Sciences, Imperial College London, London, United Kingdom.
  • Torres VL; Infection & Immunity Program, Biomedicine Discovery Institute and Department of Microbiology, Monash University, Clayton, Australia.
  • Deo P; Infection & Immunity Program, Biomedicine Discovery Institute and Department of Microbiology, Monash University, Clayton, Australia.
  • Webb CT; Infection & Immunity Program, Biomedicine Discovery Institute and Department of Microbiology, Monash University, Clayton, Australia.
  • Huang C; Monash Proteomics & Metabolomics Facility, Department of Biochemistry and Molecular Biology, Biomedicine Discovery Institute, Monash University, Clayton, Australia.
  • Schittenhelm RB; Monash Proteomics & Metabolomics Facility, Department of Biochemistry and Molecular Biology, Biomedicine Discovery Institute, Monash University, Clayton, Australia.
  • Beeby M; Department of Life Sciences, Imperial College London, London, United Kingdom.
  • Gumbart JC; School of Physics, Georgia Institute of Technology, Atlanta, United States.
  • Lithgow T; Infection & Immunity Program, Biomedicine Discovery Institute and Department of Microbiology, Monash University, Clayton, Australia.
  • Hay ID; School of Biological Sciences, The University of Auckland, Auckland, New Zealand.
Elife ; 112022 01 27.
Article en En | MEDLINE | ID: mdl-35084330
The cell envelope of Gram-negative bacteria consists of two membranes surrounding a periplasm and peptidoglycan layer. Molecular machines spanning the cell envelope depend on spatial constraints and load-bearing forces across the cell envelope and surface. The mechanisms dictating spatial constraints across the cell envelope remain incompletely defined. In Escherichia coli, the coiled-coil lipoprotein Lpp contributes the only covalent linkage between the outer membrane and the underlying peptidoglycan layer. Using proteomics, molecular dynamics, and a synthetic lethal screen, we show that lengthening Lpp to the upper limit does not change the spatial constraint but is accommodated by other factors which thereby become essential for viability. Our findings demonstrate E. coli expressing elongated Lpp does not simply enlarge the periplasm in response, but the bacteria accommodate by a combination of tilting Lpp and reducing the amount of the covalent bridge. By genetic screening, we identified all of the genes in E. coli that become essential in order to enact this adaptation, and by quantitative proteomics discovered that very few proteins need to be up- or down-regulated in steady-state levels in order to accommodate the longer Lpp. We observed increased levels of factors determining cell stiffness, a decrease in membrane integrity, an increased membrane vesiculation and a dependance on otherwise non-essential tethers to maintain lipid transport and peptidoglycan biosynthesis. Further this has implications for understanding how spatial constraint across the envelope controls processes such as flagellum-driven motility, cellular signaling, and protein translocation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de la Membrana Bacteriana Externa / Supervivencia Celular / Periplasma / Proteínas de Escherichia coli / Lipoproteínas Tipo de estudio: Prognostic_studies Idioma: En Revista: Elife Año: 2022 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de la Membrana Bacteriana Externa / Supervivencia Celular / Periplasma / Proteínas de Escherichia coli / Lipoproteínas Tipo de estudio: Prognostic_studies Idioma: En Revista: Elife Año: 2022 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Reino Unido