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SETDB1/NSD-dependent H3K9me3/H3K36me3 dual heterochromatin maintains gene expression profiles by bookmarking poised enhancers.
Barral, Amandine; Pozo, Gabrielle; Ducrot, Lucas; Papadopoulos, Giorgio L; Sauzet, Sandrine; Oldfield, Andrew J; Cavalli, Giacomo; Déjardin, Jérôme.
Afiliación
  • Barral A; DNA Repeat Lab, Institute of Human Genetics CNRS-Université de Montpellier UMR 9002, 141 rue de la Cardonille, 34000 Montpellier, France.
  • Pozo G; DNA Repeat Lab, Institute of Human Genetics CNRS-Université de Montpellier UMR 9002, 141 rue de la Cardonille, 34000 Montpellier, France.
  • Ducrot L; DNA Repeat Lab, Institute of Human Genetics CNRS-Université de Montpellier UMR 9002, 141 rue de la Cardonille, 34000 Montpellier, France.
  • Papadopoulos GL; Chromatin and Cell Biology Lab, Institute of Human Genetics CNRS-Université de Montpellier UMR 9002, 141 rue de la Cardonille, 34000 Montpellier, France.
  • Sauzet S; DNA Repeat Lab, Institute of Human Genetics CNRS-Université de Montpellier UMR 9002, 141 rue de la Cardonille, 34000 Montpellier, France.
  • Oldfield AJ; Chromatin and Splicing Lab, Institute of Human Genetics CNRS-Université de Montpellier UMR 9002, 141 rue de la Cardonille, 34000 Montpellier, France.
  • Cavalli G; Chromatin and Cell Biology Lab, Institute of Human Genetics CNRS-Université de Montpellier UMR 9002, 141 rue de la Cardonille, 34000 Montpellier, France.
  • Déjardin J; DNA Repeat Lab, Institute of Human Genetics CNRS-Université de Montpellier UMR 9002, 141 rue de la Cardonille, 34000 Montpellier, France. Electronic address: jerome.dejardin@igh.cnrs.fr.
Mol Cell ; 82(4): 816-832.e12, 2022 02 17.
Article en En | MEDLINE | ID: mdl-35081363
Gene silencing by heterochromatin plays a crucial role in cell identity. Here, we characterize the localization, the biogenesis, and the function of an atypical heterochromatin, which is simultaneously enriched in the typical H3K9me3 mark and in H3K36me3, a histone mark usually associated with gene expression. We identified thousands of dual regions in mouse embryonic stem (ES) cells that rely on the histone methyltransferases SET domain bifurcated 1 (SETDB1) and nuclear set domain (NSD)-containing proteins to generate H3K9me3 and H3K36me3, respectively. Upon SETDB1 removal, dual domains lose both marks, gain signatures of active enhancers, and come into contact with upregulated genes, suggesting that it might be an important pathway by which genes are controlled by heterochromatin. In differentiated tissues, a subset of these dual domains is destabilized and becomes enriched in active enhancer marks, providing a mechanistic insight into the involvement of heterochromatin in the maintenance of cell identity.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Heterocromatina / Histonas / Procesamiento Proteico-Postraduccional / N-Metiltransferasa de Histona-Lisina / Elementos de Facilitación Genéticos / Metilación de ADN / Ensamble y Desensamble de Cromatina / Células Madre Embrionarias de Ratones Límite: Animals Idioma: En Revista: Mol Cell Asunto de la revista: BIOLOGIA MOLECULAR Año: 2022 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Heterocromatina / Histonas / Procesamiento Proteico-Postraduccional / N-Metiltransferasa de Histona-Lisina / Elementos de Facilitación Genéticos / Metilación de ADN / Ensamble y Desensamble de Cromatina / Células Madre Embrionarias de Ratones Límite: Animals Idioma: En Revista: Mol Cell Asunto de la revista: BIOLOGIA MOLECULAR Año: 2022 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Estados Unidos