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Analysis of nucleic acids extracted from rapid diagnostic tests reveals a significant proportion of false positive test results associated with recent malaria treatment.
Hosch, Salome; Yoboue, Charlene Aya; Donfack, Olivier Tresor; Guirou, Etienne A; Dangy, Jean-Pierre; Mpina, Maxmillian; Nyakurungu, Elizabeth; Blöchliger, Koranan; Guerra, Carlos A; Phiri, Wonder P; Ayekaba, Mitoha Ondo'o; García, Guillermo A; Tanner, Marcel; Daubenberger, Claudia; Schindler, Tobias.
Afiliación
  • Hosch S; Swiss Tropical and Public Health Institute, Basel, Switzerland.
  • Yoboue CA; University of Basel, Basel, Switzerland.
  • Donfack OT; Swiss Tropical and Public Health Institute, Basel, Switzerland.
  • Guirou EA; University of Basel, Basel, Switzerland.
  • Dangy JP; Centre Suisse de Recherches Scientifiques en Côte d'Ivoire, Abidjan, Côte d'Ivoire.
  • Mpina M; Medical Care Development International, Malabo, Equatorial Guinea.
  • Nyakurungu E; Swiss Tropical and Public Health Institute, Basel, Switzerland.
  • Blöchliger K; University of Basel, Basel, Switzerland.
  • Guerra CA; Swiss Tropical and Public Health Institute, Basel, Switzerland.
  • Phiri WP; University of Basel, Basel, Switzerland.
  • Ayekaba MO; Swiss Tropical and Public Health Institute, Basel, Switzerland.
  • García GA; Ifakara Health Institute, Bagamoyo, United Republic of Tanzania.
  • Tanner M; Laboratorio de Investigaciones de Baney, Baney, Equatorial Guinea.
  • Daubenberger C; Ifakara Health Institute, Bagamoyo, United Republic of Tanzania.
  • Schindler T; Laboratorio de Investigaciones de Baney, Baney, Equatorial Guinea.
Malar J ; 21(1): 23, 2022 Jan 24.
Article en En | MEDLINE | ID: mdl-35073934
BACKGROUND: Surveillance programmes often use malaria rapid diagnostic tests (RDTs) to determine the proportion of the population carrying parasites in their peripheral blood to assess the malaria transmission intensity. Despite an increasing number of reports on false-negative and false-positive RDT results, there is a lack of systematic quality control activities for RDTs deployed in malaria surveillance programmes. METHODS: The diagnostic performance of field-deployed RDTs used for malaria surveys was assessed by retrospective molecular analysis of the blood retained on the tests. RESULTS: Of the 2865 RDTs that were collected in 2018 on Bioko Island and analysed in this study, 4.7% had a false-negative result. These false-negative RDTs were associated with low parasite density infections. In 16.6% of analysed samples, masked pfhrp2 and pfhrp3 gene deletions were identified, in which at least one Plasmodium falciparum strain carried a gene deletion. Among all positive RDTs analysed, 28.4% were tested negative by qPCR and therefore considered to be false-positive. Analysing the questionnaire data collected from the participants, this high proportion of false-positive RDTs could be explained by P. falciparum histidine rich protein 2 (PfHRP2) antigen persistence after recent malaria treatment. CONCLUSION: Malaria surveillance depending solely on RDTs needs well-integrated quality control procedures to assess the extent and impact of reduced sensitivity and specificity of RDTs on malaria control programmes.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Protozoarias / Vigilancia de la Población / Pruebas Diagnósticas de Rutina / Coinfección / Malaria / Antígenos de Protozoos Tipo de estudio: Diagnostic_studies / Incidence_studies / Observational_studies / Prognostic_studies / Risk_factors_studies / Screening_studies País/Región como asunto: Africa Idioma: En Revista: Malar J Asunto de la revista: MEDICINA TROPICAL Año: 2022 Tipo del documento: Article País de afiliación: Suiza Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Protozoarias / Vigilancia de la Población / Pruebas Diagnósticas de Rutina / Coinfección / Malaria / Antígenos de Protozoos Tipo de estudio: Diagnostic_studies / Incidence_studies / Observational_studies / Prognostic_studies / Risk_factors_studies / Screening_studies País/Región como asunto: Africa Idioma: En Revista: Malar J Asunto de la revista: MEDICINA TROPICAL Año: 2022 Tipo del documento: Article País de afiliación: Suiza Pais de publicación: Reino Unido