Your browser doesn't support javascript.
loading
Colorimetric detection of alkaline phosphatase activity based on pyridoxal phosphate-induced chromatic switch of polydiacetylene nano-liposomes.
Wang, Dong-En; You, Shangqi; Huo, Wenjing; Han, Xiang; Xu, Huiyun.
Afiliación
  • Wang DE; Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an, Shaanxi, 710072, China.
  • You S; Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an, Shaanxi, 710072, China.
  • Huo W; Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an, Shaanxi, 710072, China.
  • Han X; College of Chemistry and Chemical Engineering, Xi'an University of Science and Technology, Xi'an, Shaanxi, 710054, China.
  • Xu H; Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an, Shaanxi, 710072, China. celldon@nwpu.edu.cn.
Mikrochim Acta ; 189(2): 70, 2022 01 24.
Article en En | MEDLINE | ID: mdl-35067757
A colorimetric assay based on polydiacetylenes (PDA) nano-liposomes is reported for facile and sensitive detection of alkaline phosphatase (ALP) activity. The critical basis of this method is that the interaction of pyridoxal phosphate (PLP) with nitrogenous group functionalized PDA nano-liposomes induces distinct blue-to-red color changes of PDA nano-liposomes. In the presence of ALP, as a nature substrate, PLP is enzymatically hydrolyzed to form pyridoxal, which cannot interact with PDA nano-liposomes. As a result, the concentration of PLP is reduced and the color change of PDA nano-liposomes is retarded, which is associated with ALP level. Under optimal conditions, the proposed method showed good linear relationship with ALP activity in the range 10-200 U/L with a limit of detection of 2.8 U/L. The detection process could be vividly observed with the naked eye. Additional attempts by using the method for the evaluation of inhibitor efficiency were also achieved with satisfying results. The method was further challenged with real human serum samples, showing consistent results when compared with a commercial standard assay kit. Such simple and easy-to-use approach may provide a new alternative for clinical and biological detection of ALP.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fosfato de Piridoxal / Colorimetría / Nanoestructuras / Fosfatasa Alcalina / Polímero Poliacetilénico / Liposomas Tipo de estudio: Diagnostic_studies Idioma: En Revista: Mikrochim Acta Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Austria
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fosfato de Piridoxal / Colorimetría / Nanoestructuras / Fosfatasa Alcalina / Polímero Poliacetilénico / Liposomas Tipo de estudio: Diagnostic_studies Idioma: En Revista: Mikrochim Acta Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Austria