Your browser doesn't support javascript.
loading
Detection and enumeration of Lak megaphages in microbiome samples by endpoint and quantitative PCR.
Crisci, Marco A; Corsini, Paula M; Bordin, Nicola; Chen, Lin-Xing; Banfield, Jillain F; Santini, Joanne M.
Afiliación
  • Crisci MA; Department of Structural and Molecular Biology, Division of Biosciences, University College London, London, UK.
  • Corsini PM; Quadram Institute Bioscience, Norwich Research Park, Norwich, UK.
  • Bordin N; Department of Structural and Molecular Biology, Division of Biosciences, University College London, London, UK.
  • Chen LX; Department of Earth and Planetary Science, University of California Berkeley, Berkeley, CA, USA.
  • Banfield JF; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, USA.
  • Santini JM; Department of Earth and Planetary Science, University of California Berkeley, Berkeley, CA, USA.
STAR Protoc ; 3(1): 101029, 2022 03 18.
Article en En | MEDLINE | ID: mdl-35059650
Lak megaphages are prevalent across diverse gut microbiomes and may potentially impact animal and human health through lysis of Prevotella. Given their large genome size (up to 660 kbp), Lak megaphages are difficult to culture, and their identification relies on molecular techniques. Here, we present optimized protocols for identifying Lak phages in various microbiome samples, including procedures for DNA extraction, followed by detection and quantification of genes encoding Lak structural proteins using diagnostic endpoint and SYBR green-based quantitative PCR, respectively. For complete details on the use and execution of this protocol, please refer to Crisci et al., (2021).
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacteriófagos / Microbiota / Microbioma Gastrointestinal Tipo de estudio: Diagnostic_studies / Guideline Límite: Animals Idioma: En Revista: STAR Protoc Año: 2022 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacteriófagos / Microbiota / Microbioma Gastrointestinal Tipo de estudio: Diagnostic_studies / Guideline Límite: Animals Idioma: En Revista: STAR Protoc Año: 2022 Tipo del documento: Article Pais de publicación: Estados Unidos